Cellulose synthase is a critical catalytic subunit of the cellulose synthase complex.To study functions of CesA genes in rice,their antibodies were prepared.First,recombinant plasmids were constructed with cloning the cellulose synthase genes into the expression vector pGEX-4T-3.The recombinant plasmids were then transformed into E.coli JM109 and the recombinant proteins were obtained.The fusion proteins were purified with GST affinity chromatography system and injected to rabbit for generating polyclonal antibodies.Finally,all antibodies were identified by Western blotting analysis with specific bands and high activities.