An 1HNMR method for quantitative detecting 2,4-dichlorophenoxyacetic acid in soil with 1,3,5-benzenetricarboxylic acid（BTC） as internal standard was developed.Proton nuclear magnetic resonance spectroscopy (1HNMR) spectra were obtained in methanol and acetone-d6 with a Bruker AV 600 MHz spectrometer at 25 ℃.For each sample，128 scans were recorded with the following parameters:observing frequency (600.13 MHz)，spectra width (9 001.95 Hz)，90° pulses width (12.10 μs) ，acquisition time (3 s) and delay time (3 s).The linearity range is 30.00-200.00 mg/L,the minimum detection of 2,4-D was 25.0 mg/kg.The average recoveries of 2,4-D in paddy soil and garden soil were 83.54%-90.26% and 85.26%-92.65% with CVs of 3.82%-5.15% and 2.32%-5.89%,respectively.