In this paper, a full cDNA encoding LAC-1 from Kaburagia rhusicola Takagi was obtain by RT-PCR and RACE techniques. The complete cDNA（2 327 bp）contains a 1 773 bp open reading fram encoding 597 amino acid residues and flanked with a 449 bp 5′UTR and a 105 bp 3′ UTR. The predicted molecular weight of this complete amino acid is about 67 ku with an isoelectric point of 6.31. Compared with other insect laccase, the cloned gene shares four conserved anino acid sequences motife HWHGHH, THFWHSH, HPFHLHGH and WLFHCHIEFH. The analysis of phylogenetic relationship showed that the clonde gene has high amino acid sequences homology with other insect laccase. 