Nine strains of endophytic fungi were isolated from rapeseed and classified into 4 genera through morphological and molecular biological identification.Plate confronting test showed that the endophytic Chaetomium globosum had different inhibition on Sclerotinia sclerotiorum,Rhizoctonia solani,Botrytis cinerea and Fusarium graminearum,etc.The mechanism of antagonism C.globosum and R.solani with confrontation on slide found that C.globosum had apparent process of mycoparasitism during the confrontation with R.solani.