To develop a method for the production of avian influenza proteins in wheat as an oral vaccine,a gene encoding hemagglutinin (HA) of avian influenza H5N1 was constructed in a wheat expression vector with promoter of cereal crops. The constructed vector DNA was bombarded into immature embryos of an elite wheat cultivar Zheng 9023. Calluses induced on media supplemented with 5 mg/L phosphinothricin (PPT) were screened and transferred to media containing 4 mg/L PPT for rooting and regeneration. The PPT-resistant green plantlets derived from the rooting media were identified by PCR,and the transgenic wheat plants carrying the HA gene were obtained. Southern blot analysis of T1 transgenic plants demonstrated that the HA gene was indeed integrated into the wheat genome with a single copy integration pattern. These results provide materials for further investigating expression of avian influenza HA gene and accumulation of its protein in wheat seeds.