Collagen was extracted from grass carp scale with acid-enzyme combination-method for preparing fish scale collagen film (FCF) by the plate pouring method.The film-forming conditions and chitosan on the properties of gelatin film were studied to optimize the preparation conditions of FCF.The results showed that the conditions forming scales and polysaccharides have significant effects on the fish scale collagen membrane characteristics.The optimum conditions were 45 ℃,pH 5,collagen/chitosan=6∶4.The tensile strength was 61.27 MPa,and elongation at break was 5.17%.