To alleviate the toxic effects of heavy metals on Pleurotus ostreatus,detoxification of rice bran and Swida wilsoniana oilcake to heavy metals was studied through a series of plate cultivation and sawdust cultivation.The results showed that the mycelium growth of Pleurotus ostreatus were all significantly inhibited when the basic cultivating medium was contaminated by 50 mg/L Pb2+ or 15 mg/L Hg2+,respectively.Both of rice bran and Swida wilsoniana oilcake can promote the mycelium growth of Pleurotus ostreatus remarkably when they was added to the basic medium,and the best concentration was 20 g/L.Meanwhile,rice bran and Swida wilsoniana oilcake can take chelation on Pb2+ or Hg2+.The chelation capacity of rice bran on Hg2+ is one order of magnitude stronger than that of Swida wilsoniana oilcake.When rice bran and Swida wilsoniana oilcake are added in the medium contaminated by Pb2+ or Hg2+ respectively,the toxicity of Pb2+,Hg2+ to mycelium growth was decreased and the growth ofPleurotus ostreatus was recovered.In cultivation experiments,rice bran and Swida wilsoniana oilcake showed a very strong effects on chelate detoxification and increased yield as they were added in cultivation materials.Analyzing concentration of heavy metals in fruiting bodies of Pleurotus ostreatus by different cultivation methods,it was found that the concentration of Pb2+or Hg2+ were decreased when rice bran and Swida wilsoniana oilcake were added in corresponding cultivation materials,especially the detecting results of tests about lead conformed to the hygienic standard for edible fungi in China.