Macro-porous adsorption resins,AB-8 and ADS-17,were used to extract small chemical molecules from bacterial fermentation liquid and the absorbed small molecules were eluted with 50% ethanol.The neuraminidase inhibition activities of the extracts were determined with a sensitive fluorometric assay using the substrate sodium (2′-O-4-methylumbelliferyl N-acetylneuraminic acid,MUNANA),the reaction was carried out at pH 6.0 and in the presence of 1.0 μL of viral solution.The results showed that resin purification method is much better than using fermentation liquid directly.The small molecules can be greatly enriched and the amount of fermentation liquid required in resin purification method is much less than that of organic reagent extraction.Extracts of resin-purification method is equivalent to ethyl acetate extraction in activity against neuraminidase of influenza virus H1N1.But the cost of time and reagent is much less,indicating that the resin purification method is suitable for high throughput preparation of microbial metabolites.