The Trichogramma larvae were raised under constant high temperature (30 ℃ and 33 ℃)24 hours after being hatched and their adults were fed with tetracycline of different concentrations (tetracycline+30% honey water：1,10,100 mg/mL). The effects of the above treatment on the sex and egg production of the thelytokous parthenogenesis Trichogrammapretiosum were studied. The results showed that high temperature had a great effect on T.pretiosum, which would become arrhenotoky. It showed that high temperature can kill Wolbachia rapidly. Compared with the untreated T.pretiosum,T.pretiosum growing in high temperature had lower egg production and some intersexes (less than 1%) emerged. The effect of tetracycline on the sex and the egg production of T.pretiosum was also studied. Adults of completely thelytokous parthenogenesis T.pretiosum were continuously provided with tetracycline+30% honey water. After treatment there were some males and intersexes appearing in each generation. Most of the progeny were still females. After continuous treatment of 8 generations with different tetracycline concentrations, the mean number of eggs laid by treated T.pretiosum adults had a decrease tendency at first, and reached the least at F2. And the fecundity tended to ascend later,and the mean number of eggs at F7 and F8 was even higher than that of F1.The mean number of eggs of each generation by tetracycline treatment was distinctly less than that of the untreated(F0). 