The pathogenicity of Verticillium lecanii MZ041024 to the adults and larvae of Thrips palmi Karny under five different concentrations,3.0×104，3.0×105，3.0×106，3.0×107， 3.0×108 spores/mL,was determined. The results indicated that the pathogenicity of Verticillium lecanii MZ041024 to the mortality rate of the adults was respectively 45.85%, 51.67%, 71.49%, 76.93% and 84.66% under the concentrations of 3.0×104，3.0×105，3.0×106， 3.0×107, 3.0×108 spores/mL.After 5 d,6 d,7 d and 8 d treated,the LC50 of the adults was (2.999±0.017)×107,(2.869±0.041)×106,(3.569±0.053)×105, (8.724±0.094)×104 spores/mL,while the LT50 of the adults was (7.73±0.11) d,(5.61±0.16) d, (5.04±0.16) d and (4.59±0.09) d under the concentrations of 3.0×105，3.0×106，3.0×107 and 3.0×108 spores/mL. The mortality rate of the larvae was respectively 46.82%,54.97%,72.63%, 78.85% and 87.69% under the concentrations of 3.0×104 ，3.0×105，3.0×106，3.0×107 and 3.0×108 spores/mL. After 5 d,6 d,7 d and 8 d treated, the LC50 of the larvae was (1.123±0.007)×107, (1.199±0.018)×106, (1.873±0.017)×105, (6.682±0.041)×104 spores/mL,while the LT50of the larvae was (7.15±0.13) d,(5.48±0.14) d,(4.99±0.23) d and (4.32±0.23) d under the concentrations of 3.0×105, 3.0×106, 3.0×107 and 3.0×108 spores/mL.