The dry cell weight of T.glabrata reached to 3.5 g/L when 30 g/L of gluconate was presented in the culture medium.When 10 g/L of gluconate was supplemented to the culture broth with 90 g/L of glucose at 16 h,the dry cell weight,the rate of glucose consumption and the pyruvate production were increased by 7.96%,13.04% and 32.81%,respectively.The enhancement of glucose consumption and pyruvate production rate were due to the fact that the concentration of NAD+increased by 29.73% while the concentration of ATP and the ratio of NADH/NAD+ decreased 26.89% and 8.50%,respectively.The decrease in the intracellular NADH and ATP level decreased the inhibition effect of NADH and ATP,thus significantly increased the glycolytic ratio.