One hundred and five yeasts isolated from spontaneous fermentation of Cinsault were identified primarily on Wallerstein laboratory nutrient agar medium and classified by analyzing 26S rDNA D1/D2 domain sequence.Results showed that 105 yeasts were found to be 5 species belonging to 4 genera including Hanseniaspora uvarum,Pichia kluyveri,Candida zemplinin,Saccharomyces cerevisiae and Saccharomyces pastorianus with proportion of 3%,8%,1%,1% and 87%,respectively.The number of the non-Saccharomyces was gradually reducing from 24.7% to 3.9% and 0% during the early,immediate and late stage of spontaneous fermentation.