To develop a wheat genetic transformation system that is not restricted by seasons,this work investigated the optimized conditions and methods for induction and regeneration of multiple shoots directly derived from shoot apical meristem tissues in wheat.The study included the comparisons of three germination media,four induction media,three induction times and three rooting media with three elite wheat cultivars.The results indicated that MB5 basal medium without any hormones was suitable for germination of wheat mature embryos.MB5 basal medium plus 1.0 mg/L TDZ and 0.5 mg/L IBA produced the highest rate of multiple shoot induction.Numbers of multiple shoots increased with the increase of induction time.The suitable time for multiple shoot induction is not more than 30 days.There was no difference among the wheat cultivars in terms of induction rate and number of multiple shoots.The best medium for rooting was 1/2 MS basal medium plus 1.0 mg/L IBA.Regenerated plants derived form multiple shoots showed normal flowering and seeds.These results provide useful methods for regeneration and genetic transformation based on multiple shoots of wheat.