A novel bioflocculant HBF-3 produced by deep-sea Halomonas sp.V3a′was used to remove Congo red from simulated dye wastewater.The effects of HBF-3 concerntration,pH and temperature on the dye removal were studied.With the increasing of HBF-3 concentration,the removal rate increased,when the concentration was 100 mg/L,the removal rate to 200 mg/L Congo red was up to 83.7%.In the range of pH 5.0-6.0,the removal rate was only 41.1%,but it kept about 88.7% in the optimal pH range of 7.0-9.0.Simultaneously,HBF-3 performed well over a wide temperature (10-70℃) range.In addition,the removal efficiency of HBF-3,active carbon and chitosan were also compared.