The purpose of the experiment is to establish an evaluation method of garlic resistance to tip blight.According to the results of field investigation, the experiment collected 3 cultivars G087,G064,G039 with different disease resistance to garlic tip blight as the identification cultivars.The spore suspension concentration,the spore inoculation position and the culture temperature after inoculation and the plant age for in vitro identification and the methods for in vivo identification have been compared and screened. The investigating disease index and the cultivar resistant types were measured and the best in vitro identification results which was in agreement with the resistance level of the identification cultivars were gotten under the conditions as follows: the inoculating leaves at 4~5 leaf stage of plant, inoculating on leaf face or leaf back, inoculating spores at the concentration of 106/mL,culturing the inoculated leaves at the temperature of 21℃ for 7 days.Both in vitro and in vivo identification results were in agreementwith field disease resistance of the identification cultivars. However，the in vitro identification was simpler and faster than the in vivo identification.The established in vitro identification technology in this experiment is simple in method, short in identification cycle duration and accurate in result, which can be used to evaluate the disease resistance of garlic germplasm resources to tip blight.