Construction of Prokaryotic Vector and Expression of the CP Fragment of Tobacco ringspot virus

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Construction of Prokaryotic Vector and Expression of the CP Fragment of Tobacco ringspot virus
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The CP fragment of the Tobacco ringspot virus(TRSV) was amplified by RT-PCR,and inserted into the expression vector pET28a.The recombinant plasmid pET-CP was transformed into Escherichia coli BL21(DE3),the target protein was expressed with induction of IP

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乔艳艳,杨翠云,于翠,曹洁,马青,张丽莉. Construction of Prokaryotic Vector and Expression of the CP Fragment of Tobacco ringspot virus[J]. Jorunal of Huazhong Agricultural University,2008,27(3).

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