Study of Nested PCR System for Sex Identification of Preimplantation Bovine Embryo
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Abstract:
Two pairs of SRY gene primer were designed as the nested primers for sex identification and one pair of HBB gene primer as an internal standard in this study and established the system of nested polymerase chain reaction(nested PCR).DNA samples of 50 fema
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周木清,刘辉,李邦佑,杨利国,周世同,胡修忠,吴世清,金尔光,杨翔,童勤,王春芳,韩艳云,张淑君. Study of Nested PCR System for Sex Identification of Preimplantation Bovine Embryo[J]. Jorunal of Huazhong Agricultural University,2007,26(1).