Cloning, Sequence Analysis and Expression of B Subunit of Shiga-like Toxin IIe Gene in Escherichia coli
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S852.612 S858.28
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Abstract:
The SLT-IIeB gene without its signal and transmembrane sequence was amplified by PCR from a Escherichia colistrain called Ee responsible for the edema disease in piglets in Hubei Province.The amplicon was cloned intothe prokaryotic expressionvector pGEX-K
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林艺远,刘国平,吴斌,赵战勤,陈焕春. Cloning, Sequence Analysis and Expression of B Subunit of Shiga-like Toxin IIe Gene in Escherichia coli[J]. Jorunal of Huazhong Agricultural University,2006,25(5).