Cloning and Expression of a 6-phosphomannose Isomerase Gene from Escherichia coli
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Abstract:
A 6-phosphomannose isomerase(PMI) gene,called manA,from Escherichia coliwas cloned by PCRand used for construction of expression vectors for both prokaryotesand eukaryotes.Upon induction with IPTG a GST-PMI fusion protein was expressed in E.coliand purifi
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张倩,廖玉才,陈方方,董璇,李和平. Cloning and Expression of a 6-phosphomannose Isomerase Gene from Escherichia coli[J]. Jorunal of Huazhong Agricultural University,2006,25(5).