Expression of SARS-CoV Spike Protein Fragment of S144-643 and Its Immunogenicity
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Abstract:
The gene fragment of S144-643 was subcloned into pET-28b and expressed the protein in E.coli.The protein existed mainly in bacterial inclusion bodies.The S144-643 was purified from bacterial inclusion bodies,refolded with the refolding buffer containing r
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费小战,卢海松,郭红燕,谭亚娣,陈焕春,郭爱珍. Expression of SARS-CoV Spike Protein Fragment of S144-643 and Its Immunogenicity[J]. Jorunal of Huazhong Agricultural University,2006,25(1).