首页 > 过刊浏览>2025年第44卷第1期 >85-93. DOI:10.13300/j.cnki.hnlkxb.2025.01.009
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间作相生相克作物对香葱根系内生微生物群落的塑造特征
作者:
作者单位:

1.广西大学农学院/广西农产品安全重点实验室/植物科学国家级实验教学示范中心，南宁 530004;2.广西河池市天峨县瑶族乡农业服务中心，河池 547399

作者简介:

殷彦，E-mail：2363947287@qq.com

通讯作者:

杨尚东，E-mail： ysd706@gxu.edu.cn

中图分类号:

S633.9.01

基金项目:

广西重点研发计划项目 （桂科 AB23026076） ； 广西大学巴马产教融合研究院专项（巴人科20220005）；广西大学农博士产教融合研究院科研项目（202300109）


Characteristics of the endophytic microbial community in root system of chives shaping by intercropping allelopathic crops
Author:
Affiliation:

1.College of Agriculture， Guangxi University/Guangxi Key Laboratory of Agricultural Product Safety/ National Experimental Teaching Demonstration Center for Plant Science， Nanning 530004， China;2.Agricultural Service Center of Yao Township， Tiane County， Hechi City， Guangxi Zhuang Autonomous Region， Hechi 547399， China

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    摘要:

    为解析间作相生相克作物对香葱生长及抗性的作用机制，设置C：香葱单作处理、F：相克处理组合（香葱/豌豆间作）和H：相生处理组合（香葱/番茄间作），比较分析香葱间作不同作物对根系内生微生物群落组成的影响。结果显示：unclassified_f_Xanthomonadaceae、独岛杆菌属 （Dokdonella）、黄杆菌属 （Flavobacterium）、unclassified_f_Alcaligenaceae、 德沃斯氏菌属（Devosia）细菌是香葱间作相生相克作物体系下香葱根系排名前5的共有优势内生细菌属；同时，嗜酸菌属 （Acidovorax）细菌是相克处理组合：香葱/豌豆间作体系下， 香葱根系中特有的优势内生细菌属。单镰刀菌属 （Fusarium）、炭疽菌属 （Colletotrichum）、Plectosphaerella、unclassified_o_Helotiales、新赤壳属 （Neocosmospora）真菌是香葱间作相生相克作物体系下香葱根系排名前5的共有优势内生真菌属；赤霉属 （Gibberella）和Acrocalymma属真菌是相生处理组合香葱/番茄间作体系下香葱根系中特有的优势内生真菌属； Dactylonectria和漆斑菌属 （Myrothecium）真菌是相克组合处理（香葱/豌豆）间作体系下香葱根系中特有的优势内生真菌属。结果表明，香葱间作不同作物塑造了香葱根系相异的内生微生物群落，香葱间作相生作物体系中，香葱根系中形成了具有促进作物生长、提高作物抗性功能的内生微生物群落；而香葱间作相克作物体系中，香葱根系中形成了以病原微生物为主体的内生微生物群落，这可能是香葱间作不同作物产生相生相克现象的重要机制之一。

    Abstract:

    Treatments including C （chives monoculture）， F （intercropping chives and pea）， and H （intercropping chives and tomato） were set to analyze the mechanism of intercropping allelopathic crops on the growth and resistance of chives. The effects of intercropping different crops with chives on the composition of endophytic microbial communities in the root system of chives were compared. The results showed that unclassified_f_Xanthomonadaceae， Dokdonella， Flavobacterium， unclassified_f_Alcaligenaceae and Devosia were the top 5 dominant endophytic bacterial genera in the root system of chives under the intercropping allelopathic crops. Acidovorax bacteria were the allelopathic combination. There was the dominant endophytic bacterial genus unique to the root system of chives under F （intercropping chives and pea）. Fusarium， Colletotrichum， Plectosphaerella， unclassified_o_Helotiales， and Neocosmospora fungi were the top 5 dominant endophytic fungi genera in the root system of chives under the intercropping allelopathic crops. Gibberella and Acrocalymma fungi were the dominant endophytic fungi unique to the root system of chives under H （intercropping chives and tomato）. Dactylonectria and Myrothecium fungi were the dominant endophytic fungi unique to the root system of chives under F （intercropping chives and pea）. It is indicated that intercropping chives with different crops shaped the composition of endophytic microbial communities in the root system of chives. In the intercropping system of chives， an endogenous microbial community with the functions of promoting crop growth and enhancing crop resistance is formed in the root system of chives. In the intercropping system of chives， an endophytic microbial community dominated by pathogenic microorganisms is formed in the root system of chives， which is one of the important mechanisms for intercropping allelopathic crops with chives as well.

    图1 不同栽培模式下香葱植株根系内生细菌门分类水平群落相对分布Fig.1 Relative distribution of endophytic bacteria at phylum level in roots of chives under different cultivationmodes
    图2 不同栽培模式下香葱植株根系内生细菌属分类水平群落相对分布Fig.2 Relative distribution of endophytic bacteria at genus level in the root system of chives under different cultivation modes
    图3 不同栽培模式下香葱植株根系内生真菌门分类水平群落相对分布Fig.3 Relative distribution of endophytic fungi at phylum level iin roots of chives under different cultivation modes
    图4 不同栽培模式下香葱植株根系内生真菌属分类水平群落相对分布Fig.4 Relative distribution of endophytic fungi at genus level inin root of chives under different cultivationmodes
    表 1 测序类型与引物序列名称Table 1 Sequencing type and primer sequence
    表 2 不同栽培模式下香葱根系内生细菌物种分类统计Table 2 Taxonomic analysis of endophytic bacterial species in the rhizosphere of chives under various cultivation modes
    表 3 不同栽培模式下香葱植株根系内生细菌Alpha多样性指数Table 3 Alpha diversity index of endophytic bacterial species in the root system of chives plants under different cultivation modes
    表 4 不同栽培模式下香葱根系内生真菌物种分类统计Table 4 Classification of endophytic fungi in chive roots under different intercropping modes
    表 5 不同栽培模式下香葱植株根系内生真菌Alpha多样性指数Table 5 Alpha diversity index of endophytic fungi in roots of chives under different cultivation modes
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殷彦,周润澈,孙妍,庞良琼,鲍彩虹,陈肯,杨尚东.间作相生相克作物对香葱根系内生微生物群落的塑造特征[J].华中农业大学学报,2025,44(1):85-93

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  • 收稿日期:2024-03-15
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