Leaves of ‘Jingzao 39’were used as explants to establish an efficient genetic transformation system for ‘Jingzao 39’. The effects of the selection pressure of kanamycin （Kan）， the inhibitory concentration of cefotaxime （Cef） and timentin （Tim）， pre-culture time， the density of bacterial suspension， infection duration， the concentration and co-culture time of acetylyringone （AS） on the transformation rate of leaves of ‘Jingzao 39’ were studied. The results showed that the optimal genetic transformation conditions for the leaves of ‘Jingzao 39’ were as follows： 5 mg/L Kan， 50 mg/L Cef， 250 mg/L Tim， pre-culture for 4 days， Agrobacterium tumefaciens concentration OD_{600 nm}=0.6， infection duration of 20 minutes， 200 μmol/L AS， co-culture for 4 days. The results of fluorescence detection and PCR analyses confirmed that the exogenous target gene was successfully integrated into the genome of ‘Jingzao 39’， achieving the Agrobacterium mediated genetic transformation of leaves of ‘Jingzao 39’. It will provide an important support for analyzing the function of genes and molecular breeding in jujube.