铁锰氧化肠杆菌和丛毛单胞菌对小白菜吸收镉和砷的影响
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作者单位:

华中农业大学生命科学技术学院/农业微生物资源发掘与利用全国重点实验室,武汉 430070

作者简介:

霍雪琪,E-mail:603360546@qq.com

通讯作者:

史凯祥,E-mail:kaixiangshi@mail.hzau.edu.cn

中图分类号:

Q939

基金项目:

国家自然科学基金项目(32270110)


Effects of iron- and manganese-oxidizing Enterobacter and Comamonas on absorption of cadmium and arsenic in Brassica rapa L.
Author:
Affiliation:

College of Life Science and Technology,National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University,Wuhan 430070,China

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    摘要:

    为探究土壤中镉(cadmium)和砷(arsenic)共去除的可行方法,选取具有铁锰氧化能力的细菌肠杆菌A11和丛毛单胞菌A23,研究混合菌株介导生成生物铁锰氧化物对镉和砷的吸附效果。扫描电镜结果显示铁锰氧化物存在于细胞表面,并且铁锰氧化物使细菌聚集成团;盆栽试验结果显示A11和A23菌株能够钝化土壤中的镉和砷,降低生物可利用态镉和砷的比例,并增加其不可利用态比例,同时还抑制小白菜地上部分和根部对镉和砷的吸收与积累。以上结果表明A11和A23混合菌株生成的生物铁锰氧化物对镉和砷有良好的吸附效果。

    Abstract:

    Enterobacter sp.A11 and Comamonas sp.A23 with the ability to oxidize Fe and Mn compounds was used to study the feasible methods for co-removing the cadmium (Cd) and arsenic (As) in soil.The effects of the biological Fe and Mn oxides (BFMO) generated by the mixed strains of A11 and A23 on the absorption of cadmium and arsenic in B. rapa. The results of scanning electron microscopy (SEM) showed that iron and manganese oxides were located on the surface of cells and led bacteria to aggregate into clusters.The results of pot experiment showed that strains A11 and A23 immobilized Cd and As in soil,reduced the proportion of bioavailable Cd and As,increased the proportion of unavailable Cd and As,and exhibited the absorption and accumulation of Cd and As in the aboveground and root parts of Brassica rapa L. as well.It is indicated that the biological iron manganese oxide generated by the mixed strains of A11 and A23 has a good adsorption effect on cadmium and arsenic in B. rapa.

    表 1 小白菜盆栽试验不同处理组设置Table 1 Different treatment groups of Brassica rapa L. pot experiment
    图1 肠杆菌A11和丛毛单胞菌A23的铁氧化和锰氧化能力及共培养对镉和砷的去除效果Fig.1 The iron-oxidation and manganese-oxidation of Enterobacter sp. A11 and Comamonas sp. A23 and the immobilized ability of strains A11 and A23 for cadmium and arsenic
    图2 肠杆菌A11和丛毛单胞菌A23共培养生成铁锰氧化物的扫描电镜和能谱扫描分析Fig.2 SEM and EDX analysis of the production of Fe-Mn oxides achieved through the co-cultivation of Enterobacter sp. A11 and Comamonas sp. A23
    图3 肠杆菌A11和丛毛单胞菌A23处理土壤盆栽试验中小白菜镉和砷的含量Fig.3 The content of cadmium and arsenic of Brassica rapa L. under the Enterobacter sp.A11 and Comamonas sp. A23 treatments in soil pot experiments
    图4 肠杆菌A11和丛毛单胞菌A23处理对小白菜生长和酶活性的影响Fig.4 The growth and enzyme activities of Brassica rapa L. under the Enterobacter sp.A11 and Comamonas sp. A23 treatments
    图5 肠杆菌A11和丛毛单胞菌A23处理对根际土壤中砷的化学形态的影响Fig.5 Effect of the chemical fractions of arsenic in rhizosphere soil under the Enterobacter sp. A11 and Comamonas sp. A23 treatments
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霍雪琪,王杏,叶林繁,史凯祥.铁锰氧化肠杆菌和丛毛单胞菌对小白菜吸收镉和砷的影响[J].华中农业大学学报,2024,43(4):212-220

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  • 收稿日期:2024-05-15
  • 在线发布日期: 2024-07-26
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