The previous studies in authors’ group indicated that an endophytic Fusarium lateritium from the Nothapodytes pittosporoides （Oliv.） plant may promote the growth and disease resistance of potato.In order to expand the application scope of this strain，tomato，which is also a Solanaceae crop，was used to study the effects of F. lateritium on the growth and disease resistance.The results showed that the plant height of tomato increased by 1.15 times compared with the control group after 50 days of root-soaking treatment with F. lateritium.The content of chlorophyll a and chlorophyll b were 1.16 times and 1.47 times higher than that of the control group.The root biomass of the treatment was 1.38 times higher than that of the control，indicating that F. lateritium promotes the growth of tomato.The results of bioassay showed that the fusarium wilt disease index of tomato （45.16%） in the treatment group with F. lateritium was about 28.99% lower than that in the control group （74.15%），indicating that F. lateritium enhances the resistance of tomato to fusarium wilt.The expression patterns of key genes in the auxin synthesis，salicylic acid synthesis and jasmonic acid pathway of tomato were analyzed after 20，30 and 40 days of treatment.The results showed that the treatment affected the expression of key genes of plant hormones to different degrees.The GFP-labeled strain was further used for fluorescence colonization observation and the colonization rate was calculated by qPCR method.After 14 hours，the presence of F. hyphae was observed in the root system of tomato.The colonization rate of F. hyphae gradually increased with the extension of the treatment time within a certain period of time，indicating that F. lateritium can endogenously colonize the root system of tomato.In conclusion，it is speculated that F. lateritium can mediate the expression of phytohormone-related genes through the endogenous colonization in roots of tomato，thereby affecting the plant growth and disease resistance of tomato.