Genetic analysis and gene mapping of lobed leaf trait inradish
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摘要:
为筛选控制萝卜花叶性状的候选基因，以萝卜花叶品种J4和板叶品种WA构建F2分离群体，在F2群体中，选择20个花叶和20个板叶单株，分别构成花叶和板叶DNA混合池，分析花叶性状的遗传规律。结果显示：萝卜花叶性状受1对基因控制，且花叶对板叶呈不完全显性；结合集团分离分析（bulked segregant analysis，BSA）与简化基因组测序基因型分析技术（genotyping by sequencing，GBS），将花叶基因定位在R7染色体0.07~7.97 Mb区间内；通过萝卜全基因组与甘蓝型油菜全基因组的共线性分析发现，萝卜候选区间内0.87~1.32 Mb与油菜A10染色体的16.35~16.80 Mb存在很好的共线性；对共线性区段进行基因功能注释确认Rs390250（899 863~901 651 bp）为萝卜花叶的候选基因；该基因编码1个HD-ZipⅠ（the class I homeodomain leucine-zipper）转录因子，其位于第二外显子的非同义突变位点T425C会导致LZ（leucine zipper）结构域内保守氨基酸的改变，可能是导致叶形变异的关键位点。
Abstract:
A F2 population of radish （Raphanus sativus L.） was constructed by crossing the lobed-leaf cultivar J4 with the serrated-leaf cultivar WA.Two bulk DNA samples consisted of equivalent amounts of DNA from 20 individuals with lobed-leaf and 20 individuals with serrated-leaf in the F2 population were used to study the inheritance of the lobed-leaf in radish.The results showed that the leaf in radish was controlled by a single gene.The lobed-leaf is incomplete dominant over the serrated-leaf.The gene for lobed-leaf was initially mapped to the genomic region from 0.07 Mb to 7.97 Mb on chromosome R7 through bulked segregant analysis （BSA） and genotyping by sequencing （GBS）.It was found that there is good collinearity between 0.87-1.32 Mb in the candidate interval of radish and 16.35-16.80 Mb of the A10 chromosome of rapeseed by analyzing the collinearity between the whole genome of radish and rapeseed（Brassica napus）.Gene function annotation of the collinear segment confirmed that Rs390250（899 863-901 651 bp） is a candidate gene of lobed-leaf in radish.This candidate gene encodes a HD-ZipⅠ （the class Ⅰ homeodomain leucine-zipper） transcription factor.Its non-synonymous mutation site T425C located in the 2nd exon leads to changes in conserved amino acids in the LZ （leucine zipper） domain，indicating that it may be a key site for the variation of leaf morphology.