To explore the differential effects of boron （B），zinc （Zn），and molybdenum （Mo） on fruit quality and mineral nutrients content of the blueberry，four treatments including conventional fertilizer （CK），B，Zn and Mo fertilizer were conducted in a long-term position experiment of trace elements. The results suggested that the application of B，Zn and Mo could significantly reduce the titratable acid content of blueberry fruits，resulted in the increases of solid acid ratio of fruit by 26.28%，14.73%，and 27.56% compared with the control，respectively. The application of B，Zn and Mo significantly increased the content and accumulation of nitrogen（N），phosphorus（P），and potassium（K） in blueberry fruits，indicated that the B，Zn，and Mo were beneficial to the transfer of N，P，and K elements from plants to fruits. In addition，Zn and B treatments increased Zn accumulation by 28.95% and 21.88%，respectively. Besides，B，Zn，and Mo treatments increased Mo accumulation by 49.37%，58.86%，and 245.57%，respectively. Generally，the comprehensive score of blueberry fruit quality among all treatments was Mo>Zn>B>CK by principal component analysis，indicated that application of B，Zn，and Mo could improve the comprehensive quality of blueberry fruit，especially the Mo application.