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熊豆,谢笔钧,孙智达.甲基萘醌MK-4对类胡萝卜素在模拟胃液中氧化的保护作用[J].华中农业大学学报,2020,39(2):102-111
甲基萘醌MK-4对类胡萝卜素在模拟胃液中氧化的保护作用
Protective effect of MK-4 on oxidation of carotenoids in simulated gastric juice
投稿时间:2019-08-19  
DOI:
中文关键词:  甲基萘醌MK-4  模拟胃液  β-胡萝卜素  番茄红素  铁诱导  氧化  LC-MS  氧化产物
英文关键词:methylnaphthoquinone MK-4  simulated gastric juice  β-carotene  lycopene  ferric induced  oxidation  LC-MS  oxidation products
基金项目:湖北省农产品加工及综合利用项目(3671600302)
作者单位E-mail
熊豆 华中农业大学食品科学技术学院武汉 430070 1678941105@qq.com 
谢笔钧 华中农业大学食品科学技术学院武汉 430070  
孙智达 华中农业大学食品科学技术学院武汉 430070 sunzhida@mail.hzau.edu.cn 
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中文摘要:
      为研究甲基萘醌MK-4对类胡萝卜素(β-胡萝卜素和番茄红素)氧化的保护作用,通过模拟胃液低pH条件下铁诱导的类胡萝卜素的氧化,评价了甲基萘醌MK-4对膳食类胡萝卜素保留率的影响,并以β-胡萝卜素为例,利用液相色谱 质谱(LC MS)分别鉴定了2种不同体系(不加MK-4,加入MK-4)中β-胡萝卜素的反应产物的种类和数量。结果表明:甲基萘醌MK-4对Fe(Ⅱ)诱导的类胡萝卜素氧化的保护效果最佳,保留率可提高至原来的2倍。在胃液中,当Fe(Ⅱ)存在时,不加MK-4时,β-胡萝卜素的反应产物涉及的种类和数量最多,包括顺式异构体、环氧化合物、醛类化合物以及降解产物4类,共计10种化合物;而加入MK-4后,β-胡萝卜素的反应产物减少至7种化合物,未检测到醛类化合物及降解产物。通过对比反应前后胶束中的MK-4的高效液相色谱图发现,MK-4对β-胡萝卜素的保护作用并不是消耗其自身。
英文摘要:
      The protective effect of methylnaphthoquinone MK-4 on the oxidation of carotenoids (β-carotene and lycopene) was studied. By simulating the ferric induced oxidation of carotenoids at low pH in gastric juice,the effect of MK-4 on the retention rate of dietary carotenoids was evaluated.Taking β-carotene as an example,the type and quantity of reaction products of β-carotene in two different systems (without MK-4,adding MK-4) were identified by liquid chromatography mass spectrometry (LC-MS). The results showed that:MK 4 had the best protection effect and the retention rate could be increased by 2 times when the oxidation of carotenoids was induced by Fe(Ⅱ). For the gastric juice without MK-4 in the presence of Fe(Ⅱ),there were the most types and quantities of reaction products of β-carotene,including cis isomers,epoxy compounds,aldehydes and degradation products,a total of 10 compounds. After the addition of MK-4,the reaction products of β-carotene were reduced to 7 compounds without aldehydes and degradation products. By comparing the HPLC of MK-4 in the micelles before and after the reaction,it was found that the protective effect of MK-4 on β-carotene did not cosume itself.
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