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陈一铭,顾泽茂,李莉娟,陈天圣,袁军法.白斑综合征病毒感染对克氏原螯虾肠道菌群的影响[J].华中农业大学学报,2020,39(2):40-46
白斑综合征病毒感染对克氏原螯虾肠道菌群的影响
Effects of white spot syndrome virus (WSSV) infection on intestinal flora composition of Procambarus clarkii
投稿时间:2019-09-22  
DOI:
中文关键词:  甲壳动物; 克氏原螯虾; 肠道菌群; 白斑综合征病毒; 高通量测序  LEfSe分析; 稻田养虾
英文关键词:crustacean  Procambarus clarkii   intestinal flora  WSSV  high throughput sequencing  LEfSe analysis  shrimp in rice fields
基金项目:湖北省现代农业产业技术体系稻田综合种养创新团队和湖北省技术创新专项(2018ABA103)
作者单位E-mail
陈一铭 华中农业大学水产学院,武汉 430070 2086654718@qq.com 
顾泽茂 1.华中农业大学水产学院,武汉 430070
2.华中农业大学双水双绿研究院,武汉 430070 
 
李莉娟 华中农业大学水产学院,武汉 430070  
陈天圣 华中农业大学水产学院,武汉 430070  
袁军法 1.华中农业大学水产学院,武汉 430070
3.湖北省水生动物病害防控工程技术研究中心,武汉 430070 
jfyuan@mail.hzau.edu.cn 
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中文摘要:
      为揭示白斑综合征病毒(white spot syndrome virus,WSSV)感染克氏原螯虾(Procambarus clarkii)后对其肠道菌群的影响,运用16S rDNA基因的高通量测序技术分析健康与人工感染WSSV的克氏原螯虾的肠道菌群的组成及多样性。结果显示,感染组与对照组在肠道丰度及多样性无显著差异,但其优势菌群组成发生变化。对照组优势菌门为变形菌门(Proteobacteria)和软壁菌门(Tenericute),分别占75.42%和19.17%;感染组肠道优势菌门为变形菌门和拟杆菌门(Bacteroidetes),分别占78.14%和17.38%。利用LEfSe分析差异菌属发现,感染组与对照组相比,Candidatus bacilloplasma丰度显著减少(P<0.05),拟杆菌科(Bacteroidaceae)和黄杆菌科(Flavobacteria)的丰度显著上升(P<0.05)。此外,部分潜在病原菌如黄杆菌属(Flavobacterium)、气单胞菌属(Aeromonas)的丰度在感染后显著升高(P<0.05)。以上结果说明WSSV感染导致克氏原螯虾肠道菌群发生变化,提示WSSV或可通过干扰肠道稳态致病。
英文摘要:
      To reveal the effect of white spot syndrom virus (WSSV) infection on the intestinal flora of crayfish (Procambarus clarkii),16S high throughput sequencing technology was used to analyze the composition and diversity of the intestinal flora of crayfish in the healthy and artificial infected group.The results showed that there was no significant difference in intestinal abundance and diversity between the WSSV infected group and the control group,but the composition of their dominant flora was different.The dominant bacteria in the control group were Proteobacteria (75.42%) and Tenericute (19.17%).The dominant intestinal bacteria in the infection group were Proteobacteria (78.14% ) and Bacteroidetes (17.38%).LEfSe analysis showed that compared with the control group,the abundance of Candidatus bacilloplasma was significantly reduced (P <0.05) in the infected group,and the abundance of Bacteroidaceae and Flavobacteria was significantly increased (P<0.05).In addition,the abundance of some potential pathogens such as Flavobacterium and Aeromonas increased significantly after infection (P<0.05).These results indicated that WSSV infection caused changes in the intestinal flora of crayfish,suggesting that WSSV may cause disease by altered the homeostatic balance of the crayfish intestine.
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