Abstract:The aim of this study was to elucidate the mechanism of glucose and reproduction hormones regulating the reproductive follicles through correlation analysis of glucose,reproduction hormones (FSH,LH and 17β-E2) and their receptor genes expression in ovine follicles of different diameter follicles. Sample follicles were placed in three groups according to their diameter:small (S:2.0-3.4 mm),medium (M:3.5-5.4 mm) and large (L:＞5.5 mm),and follicles fluid and granulose cells (GCs) in each group were collected. The apoptosis of GCs in different antrum follicles was detected by annextinV-FITC,the content of glucose,FSH,LH and 17β-E2 in small,medium and large follicles was detected by radioimmunoassay,and the expression of FSHR,LHR and G6Pase in GCs from different antrum follicles were determined by qRT-PCR technique. There was no significant difference in granulosa cell apoptosis (P＞0.05) among groups,while with the increasing of ovine follicle diameter,the content of glucose,FSH,LH and 17β-E2 in follicular fluid were increased,and the transcription levels of FSHR,LHR,and G6Pase genes were extremely significantly increased in GCs (P＜0.01). There was a significant positive correlation between glucose content and FSH in different follicles,and only a significant positive correlation with LH content in large follicles (P＜0.05). In addition,the glucose content was significantly positively correlated with the expression of FSHR and G6Pase genes,and significantly positively correlated with the expression of LHR in only large follicles (P＜0.05). With the growth of ovine follicles,the content of glucose,reproductive hormones and their expression of related receptor genes were significantly correlated,suggesting that they were involved in the regulation of follicular development.