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刘春洁,栗瑞兰,宋慧子,王兆琛,杜炜,张家新.绵羊腔卵泡中葡萄糖和生殖激素及其受体基因表达的相关性分析[J].华中农业大学学报,2020,39(1):144-150
绵羊腔卵泡中葡萄糖和生殖激素及其受体基因表达的相关性分析
Correlation analysis between glucose,reproduction hormones and their receptor expression on antrum in ovine follicles
投稿时间:2019-02-19  
DOI:
中文关键词:  绵羊  腔卵泡  葡萄糖  生殖激素  受体基因  颗粒细胞  卵泡发育
英文关键词:ovine  antrum follicles  glucose  reproduction hormones  receptor genes  granulosa cells  follicular development
基金项目:国家自然科学基金项目(31460598)
作者单位E-mail
刘春洁 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018 guilt369@163.com 
栗瑞兰 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018  
宋慧子 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018  
王兆琛 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018  
杜炜 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018  
张家新 内蒙古自治区动物遗传育种与繁殖重点实验室/内蒙古农业大学动物科学学院呼和浩特 010018 zjxcau@163.com 
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中文摘要:
      通过分析绵羊不同直径腔卵泡中葡萄糖与生殖激素(FSH、LH和17β-E2)含量和相关受体基因表达的相关性,阐明不同腔卵泡发育中葡萄糖和生殖激素对卵泡生殖调控的机制。根据绵羊腔卵泡不同直径划分为小卵泡组(2.0~3.4 mm)、中卵泡组(3.5~5.4 mm)和大卵泡组(>5.5 mm)3组,收集其卵泡液及颗粒细胞(GCs)。采用AnnextinV-FITC测定小、中和大卵泡中GCs凋亡情况;利用放射免疫法检测小、中和大卵泡组中葡萄糖浓度及FSH、LH和17β-E2的含量;运用qRT-PCR技术检测小、中和大卵泡GCs中FSHR、LHR和G6Pase基因的转录水平。结果显示,不同直径绵羊腔卵泡中GCs凋亡无显著差异(P>0.05);随着腔卵泡直径的增大,卵泡液中葡萄糖浓度、FSH、LH和17β-E2的含量均升高;同时,GCs上FSHR、LHR、G6Pase基因的转录水平极显著升高(P<0.01)。不同直径腔卵泡中,葡萄糖浓度与FSH含量均呈显著正相关,仅在大卵泡中与LH呈显著正相关(P<0.05)。另外,葡萄糖浓度与FSHR、G6Pase基因的表达量呈显著正相关,只在大卵泡中与LHR表达量呈显著正相关(P<0.05)。随着绵羊腔卵泡生长,葡萄糖与生殖激素含量和相关受体基因表达量具有显著相关性,共同参与调节卵泡发育。
英文摘要:
      The aim of this study was to elucidate the mechanism of glucose and reproduction hormones regulating the reproductive follicles through correlation analysis of glucose,reproduction hormones (FSH,LH and 17β-E2) and their receptor genes expression in ovine follicles of different diameter follicles. Sample follicles were placed in three groups according to their diameter:small (S:2.0-3.4 mm),medium (M:3.5-5.4 mm) and large (L:>5.5 mm),and follicles fluid and granulose cells (GCs) in each group were collected. The apoptosis of GCs in different antrum follicles was detected by annextinV-FITC,the content of glucose,FSH,LH and 17β-E2 in small,medium and large follicles was detected by radioimmunoassay,and the expression of FSHR,LHR and G6Pase in GCs from different antrum follicles were determined by qRT-PCR technique. There was no significant difference in granulosa cell apoptosis (P>0.05) among groups,while with the increasing of ovine follicle diameter,the content of glucose,FSH,LH and 17β-E2 in follicular fluid were increased,and the transcription levels of FSHR,LHR,and G6Pase genes were extremely significantly increased in GCs (P<0.01). There was a significant positive correlation between glucose content and FSH in different follicles,and only a significant positive correlation with LH content in large follicles (P<0.05). In addition,the glucose content was significantly positively correlated with the expression of FSHR and G6Pase genes,and significantly positively correlated with the expression of LHR in only large follicles (P<0.05). With the growth of ovine follicles,the content of glucose,reproductive hormones and their expression of related receptor genes were significantly correlated,suggesting that they were involved in the regulation of follicular development.
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