• 首页|编委会|期刊简介|期刊荣誉|投稿指南|联系我们|过刊浏览
张真,梁旭方,徐晶,何珊,李姣.PYY组织表达及对翘嘴鳜的摄食调控[J].华中农业大学学报,2020,39(1):136-143
PYY组织表达及对翘嘴鳜的摄食调控
Tissue expression and food intake attenuation effect of peptide YY in Chinese perch (Siniperca chuatsi)
  
DOI:
中文关键词:  翘嘴鳜  脑肠肽  高度保守  摄食调控  食欲抑制
英文关键词:Chinese perch  braingut peptide  highly conservative  food intake regulation  appetite suppression
基金项目:国家现代农业产业技术体系专项(CARS-46);国家重点研发计划——蓝色粮仓项目(2018YFD0900400)
作者单位E-mail
张真 华中农业大学水产学院/华中农业大学鳜鱼研究中心/农业农村部鳜鱼育种创新基地/农业农村部淡水生物繁育重点实验室武汉 430070 384902947@qq.com 
梁旭方 华中农业大学水产学院/华中农业大学鳜鱼研究中心/农业农村部鳜鱼育种创新基地/农业农村部淡水生物繁育重点实验室武汉 430070 xufang_liang@hotmail.com 
徐晶 华中农业大学水产学院/华中农业大学鳜鱼研究中心/农业农村部鳜鱼育种创新基地/农业农村部淡水生物繁育重点实验室武汉 430070  
何珊 华中农业大学水产学院/华中农业大学鳜鱼研究中心/农业农村部鳜鱼育种创新基地/农业农村部淡水生物繁育重点实验室武汉 430070  
李姣 华中农业大学水产学院/华中农业大学鳜鱼研究中心/农业农村部鳜鱼育种创新基地/农业农村部淡水生物繁育重点实验室武汉 430070  
摘要点击次数: 29
全文下载次数: 33
中文摘要:
      以肉食性鱼类翘嘴鳜 (Siniperca chuatsi) 为研究对象,对其肽YY(peptide YY,PYY)基因进行鉴定和分析,并通过中枢注射人工合成的鳜PYY多肽,研究PYY对翘嘴鳜摄食量的影响。氨基酸序列多重比对结果发现,sc-PYY与各个物种的氨基酸序列高度保守,鱼类中与狼鲈(Dicentrarchus labrax)、红鳍东方鲀(Takifugu rubripes)一致性较高;实时荧光定量PCR检测sc-PYY mRNA表达的组织分布,结果显示,sc-PYY mRNA在脑中表达量最高,在肠道、肌肉、眼睛中亦有较高的表达;翘嘴鳜中枢注射PYY后,摄食量在4 h相比于对照组(PBS)显著性下降。这表明PYY可能在减少鳜的食物摄入量中发挥着重要的作用。
英文摘要:
      As the widely commercial Chinese perch species in China,the unique feeding habits and feeding regulation mechanism of Chinese perch have always been the focus of many scientific researchers. Taking Siniperca chuatsi,a carnivorous fish,as the research object,the peptide YY (PYY) gene was analyzed and identified,and the effect of PYY polypeptide on feeding of Chinese perch was studied by intracerebroventricular (icv) administration. The results showed that the full length cDNA of Chinese perch PYY (sc-PYY) encodes a precursor protein that consists of a putative 28-amino acid signalpeptide,a 36-amino acid mature peptide,an amidation-proteolytic site,and a 30-amino acid carboxy-terminalextension. The sc-PYY gene is comprised of 4 exons interspaced by 3 introns. Amino acid alignment and gene structure comparison indicated that the amino acid sequences of PYY are highly conserved. Among the fishes studied,the consistency of sc-PYY with that of seabass and Japanese pufferfish was higher. Tissue distribution and postprandial changes of the sc-PYY mRNA expression were evaluated by real-time PCR,which showed that the sc-PYY mRNA had the highest expression in the brain,followed by high expression in the intestinal tract,muscles and eyes. The PYY icv results showed that after icv treatment,the food intake of PYY injection group decreased significantly at 4 h compared with control group (PBS). This indicated that PYY might play an important role in the regulation of food intake of Chinese perch.
查看全文  查看/发表评论  下载PDF阅读器
关闭
版权所有:华中农业大学学报(自然科学版)  
主管单位:华中农业大学 主办单位:华中农业大学 地址:湖北武汉南湖狮子山华中农业大学内
电话:027-87287364 电子邮件:hnlkxb@mail.hzau.edu.cn
您是本站第8094233位访问者  今日一共访问1183次  
技术支持:北京勤云科技发展有限公司