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陈静,SHALABY Hany said ali,易少奎,王卫民.团头鲂、鳡及其杂交F1代的形态差异与系统发育研究[J].华中农业大学学报,2020,39(1):116-125
团头鲂、鳡及其杂交F1代的形态差异与系统发育研究
Morphological differences and phylogenetic analysis of Megalobrama amblycephala,Elopichthys bambusa and their F1 hybrids
投稿时间:2019-06-30  
DOI:
中文关键词:  团头鲂    远缘杂交  亚科杂交  形态差异  系统发育  线粒体基因组  鲤科鱼类
英文关键词:Megalobrama amblycephala  Elopichthys bambusa  distant hybridization  subfamily hybridization  morphological differences  phylogenetic development  mitochondrial genome  Cyprinidae
基金项目:国家自然科学基金项目(31772901)
作者单位E-mail
陈静 华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室武汉 430070 cjing24511@163.com 
SHALABY Hany said ali 华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室武汉 430070  
易少奎 华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室武汉 430070  
王卫民 华中农业大学水产学院/农业动物遗传育种与繁育教育部重点实验室/农业农村部淡水生物繁育重点实验室武汉 430070 wangwm@mail.hzau.edu.cn 
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中文摘要:
      利用不同亚科的雌性团头鲂(Megalobrama amblycephala)和雄性鳡(Elopichthys bambusa) 进行人工杂交获得子一代杂交个体,对团头鲂、鳡及其杂交子代的形态特征进行比较和分析,并对团头鲂(♀) × 鳡(♂)的线粒体全基因组序列进行扩增和分析。结果显示,在传统可量性状和框架结构中,杂交F1的性状更加偏向于母本团头鲂。在可数性状上,杂交子代的背鳍棘数、胸鳍棘数、腹鳍棘数和臀鳍棘数与母本团头鲂及父本鳡都一致;而其余大多数性状,如背鳍鳍条数、腹鳍鳍条数、臀鳍鳍条数、侧线鳞数、侧线上鳞数、侧线下鳞数和鳔室数,杂交子代的性状更接近于母本团头鲂。杂交鱼的线粒体基因组序列全长为16 623 bp,与母本团头鲂一致,其碱基组成为31.23% A、16.19% G、24.69% T和27.90% C,JP2〗且具有一定的A+T偏向性。其线粒体基因组共编码37个基因,分别为13个蛋白质编码基因、22个tRNA基因和2个rRNA基因,还有1个非编码区(D-Loop)。系统发育树结果显示,团头鲂(♀) × 鳡(♂)与母本团头鲂的亲缘关系较近,与父本鳡的亲缘关系较远。 
英文摘要:
      In this study,the inter-subfamily hybridization of female Megalobrama amblycephala and male Elopichthys bambusa was conducted to obtain the F1 hybrid offspring. Morphological characters of M. amblycephala,E. bambusa and their hybrid offspring were compared and analyzed. The results showed that most morphometric characters of the hybrid fish were closer to those of female parent M. amblycephala. Meristic characters,including the dorsal fin spines,pectoral fin spines,ventral fin spines and anal fin spines of the hybrid fish were consistent with those of both female and male parent. However,for the other characters,such as dorsal fin rays,ventral fin rays,anal fin rays,scale number on lateral line,transversal scales (upper) number,transversal scales (lower) number and swim bladder sacs,the hybrid fish were closer to female parent M. amblycephala than male parent E. bambusa. In addition,we amplified and analyzed the complete mitochondrial genome sequence of M. amblycephala (♀) ×E. bambusa (♂),which is 16 623 bp in length. The overall nucleotide composition was 31.23% A,16.19% G,24.69% T and 27.90% C,with an A+T content of 55.92%. The whole mitogenome contained 13 protein-coding genes,2 rRNA,22 tRNA,and a D-loop region. Phylogenetic analysis showed that M. amblycephala (♀) × E. bambusa (♂) had closer relationship with female parent M. amblycephala than male parent E. bambusa.
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