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吴德东,张国财,杨璟,曾建勇,张国珍.北乌头提取物对舞毒蛾体内酶活性的影响[J].华中农业大学学报,2020,39(1):95-102
北乌头提取物对舞毒蛾体内酶活性的影响
Effects of extract from Aconitum kusnezofKG-fii Rchb.on enzyme activity in Lymantria dispar
投稿时间:2019-06-10  
DOI:
中文关键词:  北乌头  提取物  舞毒蛾  解毒酶  保护酶  消化酶  酶活性  植物源杀虫剂
英文关键词:Aconitum kusnezoffii Rchb.  extract  Lymantria dispar  detoxification enzyme  protective enzyme  digestive enzyme  enzyme activity  botanical insecticide
基金项目:中央财政林业科技推广示范资金项目(\[2019\]HZT02);哈尔滨市应用技术研究与开发项目(2016RAXXJ035)
作者单位E-mail
吴德东 东北林业大学林学院哈尔滨 150040 wudedong1978@126.com 
张国财 东北林业大学林学院哈尔滨 150040 zhang640308@126.com 
杨璟 东北林业大学林学院哈尔滨 150040  
曾建勇 东北林业大学林学院哈尔滨 150040  
张国珍 东北林业大学林学院哈尔滨 150040  
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中文摘要:
      采用系统溶剂法对北乌头甲醇提取物进行分离,用饲料混药法测定4种萃取物对舞毒蛾的胃毒毒力,测定了北乌头乙酸乙酯萃取物对舞毒蛾幼虫体内解毒酶、保护酶、消化酶活力的影响。试验结果表明:北乌头乙酸乙酯萃取物杀虫作用显著,为主要杀虫活性物质;舞毒蛾幼虫经北乌头乙酸乙酯萃取物亚致死浓度(LC20)处理后,其体内羧酸酯酶、谷胱甘肽S-转移酶和乙酰胆碱酯酶活性均表现为持续抑制,处理72 h,乙酰胆碱酯酶活力为对照的50.6%,抑制作用极显著(P<0.01);超氧化物歧化酶、过氧化氢酶、过氧化物酶活性均表现为先激活后抑制,处理24 h,过氧化氢酶活力为对照的1.613倍,激活作用极显著(P<0.01),处理72 h,超氧化物歧化酶活力为对照的84.2%,抑制作用极显著(P<0.01);脂肪酶和淀粉酶活性均表现为持续抑制,处理72 h,脂肪酶活力为对照的53.9%,抑制作用极显著(P<0.01);蛋白酶活性表现为先激活后抑制,处理24 h,蛋白酶活力为对照的1.431倍,激活作用极显著(P<0.01),处理72 h,蛋白酶活力为对照的90.4%,抑制作用显著(P<0.05)。综上,北乌头乙酸乙酯萃取物可影响舞毒蛾幼虫的解毒酶、保护酶及消化酶,扰乱其正常生理代谢。
英文摘要:
      The methanol extract from the Aconitum kusnezoffii Rchb. was isolated with system solvent method and the stomach toxicity of the four extracts on Lymantria dispar was determined with feed toxicity method. Then the effects of ethyl acetate extract from A. kusnezoffii on activities of the detoxification enzyme,protective enzyme and digestive enzyme in Lymantria dispar larvae were studied. The results showed that ethyl acetate extract from A. kusnezoffii had obvious insecticidal activity against gypsy moth and was the main insecticidal active substance. After treated with ethyl acetate extract from A. kusnezoffii at a dose of sub-concentration (LC20),the activities of carboxylesterase,glutathione S-transferases and acetylcholinesterase in the body showed continuous inhibition. The inhibition rate of acetylcholinesterase after 72 h treatment was 50.6% of the control,which was extremely significant (P<0.01). The activities of superoxide dismutase,catalase,and peroxidase were first activated and then inhibited. The activation rate of catalase was extremely significant (P<0.01) at 24 h treatment,which was 1.613 times of the control. The inhibition rate of superoxide dismutase at 72 h treatment was 84.2% of the control,which was also extremely significant (P<0.01). The activities of lipase and amylase were consistently inhibited,with the inhibition rate of lipase at 72 h of 53.9% of the control,which was extremely significant (P<0.01). The protease activity was inhibited after activation,with the activation at 24 h treatment of 1.431 times of the control,which was extremely significant (P<0.01). The protease activity was inhibited at 72 h with 90.4% of the control,which was significant (P<0.05). It is indicated that ethyl acetate extract from A. kusnezoffii affects detoxification enzymes,protective enzymes and digestive enzymes of gypsy moth larvae,and disturbs its normal physiological metabolism.
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