• 首页|编委会|期刊简介|期刊荣誉|投稿指南|联系我们|过刊浏览
王丰,任灵玲,安婷婷,刘灵芝.长期施肥对土壤中氨氧化细菌丰度和种群多样性的影响[J].华中农业大学学报,2020,39(1):86-94
长期施肥对土壤中氨氧化细菌丰度和种群多样性的影响
Effect of long-term fertilization and seasonal variation on abundance and population diversity of soil ammonia oxidizing bacteria
投稿时间:2019-05-27  
DOI:
中文关键词:  长期施肥  土壤细菌  氨氧化细菌  种群结构  变性梯度凝胶电泳  氨氧化细菌丰度  季节变化; 定位施肥
英文关键词:long-term fertilization  soil bacteria  ammonia oxidizing bacteria  community structure  denaturing gel gradient electrophoresis  AOB abundance  seasonal variation  localized fertilizer application
基金项目:国家自然科学基金项目(31101504);国家重点研发项目(2017YFD0200604); 辽宁省高等学校基本科研项目(LSNZD201705)
作者单位E-mail
王丰 沈阳农业大学土地与环境学院/土肥资源高效利用国家工程实验室/农业农村部东北耕地保育重点实验室沈阳 110866 64066604@qq. com 
任灵玲 沈阳农业大学土地与环境学院/土肥资源高效利用国家工程实验室/农业农村部东北耕地保育重点实验室沈阳 110866  
安婷婷 沈阳农业大学土地与环境学院/土肥资源高效利用国家工程实验室/农业农村部东北耕地保育重点实验室沈阳 110866  
刘灵芝 沈阳农业大学土地与环境学院/土肥资源高效利用国家工程实验室/农业农村部东北耕地保育重点实验室沈阳 110866 liulingzhi2006@syau.edu.cn 
摘要点击次数: 26
全文下载次数: 36
中文摘要:
      为探讨长期施肥对土壤氨氧化细菌 (ammonia-oxidizing bacteria,AOB)的影响机制,以长期定位试验站为平台,采用变性梯度凝胶电泳 (PCR-DGGE) 技术,研究不同施肥方式下土壤AOB丰度和种群结构多样性的季节变化特征。结果表明:与不施肥相比,施肥后土壤铵态氮和硝态氮含量增加,土壤pH降低;随采样时期变化(4月、7月和11月),土壤AOB丰度和多样性指数均在4月最高;各施肥处理下,增施无机氮肥可降低土壤中总细菌数量和AOB的种群多样性,有机/无机氮肥配施有助于维持总细菌和AOB丰度的稳定;冗余分析(RDA)结果表明,铵态氮显著影响土壤AOB种群结构组成,季节是导致AOB种群结构差异的重要因素。总之,季节变化与长期定位施肥引起的土壤理化特征差异,导致土壤AOB丰度和种群多样性发生改变。与无机氮肥相比,有机/无机氮肥配施有助于稳定土壤AOB的丰度。
英文摘要:
      Based on the long-term fertilization experiment station in Shenyang Agriculture University,denaturing gradient gel electrophoresis (PCR-DGGE) was used to explore the dynamics of soil physicochemical properties and the abundance and diversity of ammonia oxidizing bacteria (AOB) with seasons under different fertilization treatments,including no fertilizer (CK),low level of nitrogen (N) fertilizer (N2),high level of N fertilizer (N4) and organic fertilizer combined with inorganic N fertilizer (M2N2). The purpose of this paper is to discuss the effects of long term fertilization on the AOB and provide the theoretical foundations for rational fertilization in this region. The results showed that,compared with CK treatment,the application of organic or inorganic fertilizers increased the contents of soil ammonium N (NH4+-N) and nitrate N (NO3--N) and decreased the soil pH value. The AOB abundance of soil sampled in April was the highest among the 3 sampled time points (April,July and November). The use of inorganic N fertilizer decreased the total bacterial abundance and AOB diversity,while the organic fertilizer combined with inorganic fertilizer would contribute to the stability of soil total bacterial abundance. Redundant analysis(RDA) revealed that NH4+-N significantly affected the population diversity of AOB,and the seasonal variation played an important role in shaping AOB population structure. Generally,the seasonal variation and the long-term fertilization regime caused the changes in soil characteristics,which led to the difference in the quantity and community structure of AOB. Compared with inorganic N fertilizer,organic fertilizer combined with inorganic fertilizer would contribute to the stability of soil AOB abundance.
查看全文  查看/发表评论  下载PDF阅读器
关闭
版权所有:华中农业大学学报(自然科学版)  
主管单位:华中农业大学 主办单位:华中农业大学 地址:湖北武汉南湖狮子山华中农业大学内
电话:027-87287364 电子邮件:hnlkxb@mail.hzau.edu.cn
您是本站第8094219位访问者  今日一共访问1175次  
技术支持:北京勤云科技发展有限公司