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刘园,向思敏,王江波,吴翠云,唐章虎,龚涵,张雪,徐娟.库尔勒香梨挥发性物质及初生代谢物的GC-MS分析[J].华中农业大学学报,2020,39(1):44-52
库尔勒香梨挥发性物质及初生代谢物的GC-MS分析
GC-MS analyses of volatiles and primary metabolites in Korla Pear fruit
  
DOI:
中文关键词:  库尔勒香梨  顶空-固相微萃取  气相色谱-质谱联用  挥发性物质  初生代谢物  果实品质  果实风味  品质评价
英文关键词:Korla Pear (Pyrus sinkiangensis Yü)  headspace-solid phase microextraction  gas chromatography-mass spectrometry  volatiles  primary metabolites  furit quality  fruit aroma  quality evaluation
基金项目:兵团南疆重点产业发展支撑计划(2017DB006-2)
作者单位E-mail
刘园 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院武汉430070 uiyuer@webmail.hzau.edu.cn 
向思敏 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院武汉430070  
王江波 南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室/塔里木大学植物科学学院阿拉尔843300新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室阿拉尔843300  
吴翠云 南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室/塔里木大学植物科学学院阿拉尔843300新疆生产建设兵团塔里木盆地生物资源保护与利用重点实验室阿拉尔843300  
唐章虎 新疆农业科学院轮台国家果树资源圃轮台841600  
龚涵 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院武汉430070  
张雪 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院武汉430070  
徐娟 园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院武汉430070 xujuan@mail.hzau.edu.cn 
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中文摘要:
      分别采用甲基叔丁基醚(MTBE)萃取法和顶空固相微萃取(HS-SPME)法提取成熟期(盛花后180 d)库尔勒香梨果实的挥发性物质,结合气相色谱-质谱联用技术(GC-MS)对挥发性物质进行定性和定量分析,在筛选适宜的挥发性物质提取方法的基础上,比较果实不同组织挥发性物质的组成差异。结果表明:相较于MTBE萃取,HS-SPME对库尔勒香梨挥发性物质有更好的萃取效果。MTBE-GC-MS法在库尔勒香梨中共检测到15种挥发性物质,未检测到酯类、芳烃及酮类物质;HS-SPME-GC-MS法共检测到98种化合物,主要为醛类、酯类、醇类以及萜烯类,(E)-2-己烯醛、己醛、α-法尼烯、1-壬醇、乙酸己酯是其主要挥发性物质;果皮比果肉含有更丰富的挥发性物质,32种物质为果皮所特有。进一步利用GC-MS对果肉中初生代谢物质进行分析,共检测到果糖、葡萄糖、山梨醇等共17种糖以及苹果酸等共8种酸,糖酸比为71.49。
英文摘要:
      The volatiles of mature (180 days after full bloom) Korla Pear fruit were extracted with methyl tert-butyl ether (MTBE) and headspace solid phase microextraction (HS-SPME),respectively. Qualitative and quantitative analyses of volatiles were performed in combination with gas chromatography-mass spectrometry (GC-MS). The composition of volatiles in different tissues was compared with the basis of screening suitable volatiles extraction methods. The results showed that the effect of extracting volatiles from Korla Pear with HS-SPME was better than that of MTBE. 15 compounds were detected with MTBE-GC-MS,while no esters,aromatic hydrocarbons nor ketones were detected. 98 compounds mainly including aldehydes,esters,alcohols and terpenes were detected with HS-SPME-GC-MS. (E)-2-hexenal,hexanal,α-farnesene,1-nonanol,hexyl acetate were the main volatiles. Volatiles in the peel were more than that in the pulp. 32 compounds were unique in the peel. The results of the primary metabolites analyzed with GC-MS showed that 17 sugars including fructose,glucose and sorbitol,and 8 organic acids such as malic acid were detected in the pulp. The sugar/acid ratio was 71.49.
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