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冯洁,曹琳琳,王越,王力明,苗佳琪,杜鹃,柳俊,蔡兴奎.无糖组织培养技术在马铃薯种苗快繁中的应用[J].华中农业大学学报,2019,38(6):-69
无糖组织培养技术在马铃薯种苗快繁中的应用
Application of sugar free micropropagation on potato seedlings rapid propagation
投稿时间:2019-05-23  
DOI:
中文关键词:  无糖组织培养  马铃薯种苗  试管苗  扦插  快繁  微型薯生产
英文关键词:sugar free micropropagation  potato seedlings  test tube seedlings  cutting  rapid propagation  minituber production
基金项目:国家马铃薯产业技术体系专项(CARS 09 P08); 湖北省科技创新重大项目(2017ABA145)
作者单位E-mail
冯洁 华中农业大学生命科学技术学院/农业农村部马铃薯生物学与生物技术重点实验室武汉 430070+T49 905468166@qq.com 
曹琳琳 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
王越 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
王力明 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
苗佳琪 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
杜鹃 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
柳俊 华中农业大学生命科学技术学院/农业农村部马铃薯生物学与生物技术重点实验室武汉 430070  
蔡兴奎 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070 caixingkui@mail.hzau.edu.cn 
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中文摘要:
      为提高马铃薯试管苗质量,优化种苗生产,改良马铃薯脱毒种薯繁育技术体系,采用无糖组织培养技术,以马铃薯早熟品种“华薯1号”的脱毒试管苗为材料,以MS培养基附加4%蔗糖培养基为对照(CK),比较无糖培养技术对试管苗生长发育的影响,并探究无糖组培下不同培养基、支撑物及接种方式对马铃薯植株生长发育的影响。结果表明:与传统的组织培养相比,无糖组织培养下试管苗生长速度快,试管苗更健壮,在马铃薯脱毒种苗生产上更具优势。无糖组织培养条件下,去除MS培养基中的有机成分,仅包含无机成分,对脱毒种苗的生长无显著影响,但更能有效降低污染。以蛭石为支撑物代替琼脂的试验结果显示,以蛭石为支撑的脱毒种苗生长速度快,节间较长,有“徒长”迹象。在以蛭石为支撑物培养条件下,比较单双节段的扦插试验结果显示,双节段扦插的种苗生长状况显著优于单节段扦插,且双节段扦插成活率更高,节段较大便于机械手操作。进一步将无糖组织培养试管苗扦插至网室生产微型薯,与传统的有糖培养试管苗相比,两者微型薯的结薯数量和大小均无显著差异。本研究建立的新型无糖组织培养技术,可缩短马铃薯种苗的培养周期,降低生产成本,简化微型薯生产扦插繁育程序,为实现马铃薯种苗机械化扦插奠定了基础。
英文摘要:
      A new technology called sugar free tissue culture technology was used to increase the quality of potato test tube seedlings and optimize the production,and to improve the technical system of virus free seed potato breeding. The virus free test tube seedlings of early maturing potato cultivar “Huashu 1” and the traditional MS medium with 4% sucrose was used as the test and control (CK) to compare two different culture methods. The effects of different media,supports and inoculation methods on the growth and development of potato plants in sugar free tissue culture were studied. The results showed that the test tube seedlings under sugar free tissue culture grew faster and were more robust,and had more advantages in the production of virus free potato seedlings compared with the traditional tissue culture. Under sugar free tissue culture conditions,further removal of organic components from MS medium,only containing inorganic components,had no significant impact on the growth of virus free seedlings,but more effective in reducing pollution. The results of using vermiculite as support instead of agar showed that the virus free seedlings supported by vermiculite grew faster,had longer internodes and showed signs of “elongation”. The results under the condition of vermiculite as support culture showed that the seedling growth of double segment cutting was significantly better than that of single segment cutting. The survival rate of double segment cutting was higher,and the segment was larger and easy to operate. The test tube seedlings under sugar free tissue culture were further cutting into the net room to produce mini tubers,of which the number and size had no significant changes compared with that from test tube seedlings under the traditional sugar culture. The new sugar free tissue culture technology shortens the cultivation period of potato seedlings,reduces the production cost,simplifies the cutting propagation procedure of minituber production. It will lay a foundation for the realization of mechanized cutting of potato seedlings.
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