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田静,庞一波,陈嘉景,袁子彧,曾继吾,徐娟.化州柚种质资源的SSR分析及其果实不同发育期柚皮苷含量变化[J].华中农业大学学报,2019,38(5):64-70
化州柚种质资源的SSR分析及其果实不同发育期柚皮苷含量变化
SSR analyses of germplasm resources and changes of naringin content at different developmental stages of Citrus grandis ‘Tomentosa’ fruit
投稿时间:2019-02-27  
DOI:
中文关键词:  化州柚  化橘红  SSR  柚皮苷  种质鉴别  适采期  高效液相色谱(HPLC)
英文关键词:Citrus grandis Osbeck var. Tomentosa Hort.  Exocarpium Citri Grandis  simple sequence repeat (SSR)  naringin  germplasm identification  optimal period for harvesting  high performance liquid chromatography (HPLC)
基金项目:国家自然科学基金项目(31521092); 农业部公益性行业(农业)科研专项(201303093)
作者单位E-mail
田静 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070 tianjing880226@163.com 
庞一波 浙江省台州市农业科学研究院临海 317000  
陈嘉景 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
袁子彧 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070  
曾继吾 广东省农业科学院果树研究所广州 510640 jiwuzeng@163.com 
徐娟 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室武汉 430070 xujuan@mail.hzau.edu.cn 
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中文摘要:
      采用SSR分子标记技术研究化州柚不同品系及柑橘属各代表种质间的亲缘关系,结果表明:25对SSR引物在15个样品中扩增得到175个等位基因,聚类分析证实化州柚与柚类亲缘关系较近,而且一些化州柚同名材料在遗传上存在差异。利用高效液相色谱(HPLC)法检测不同种质幼果及不同果径果实不同组织中柚皮苷含量,结果表明:化州柚幼果中柚皮苷含量显著高于化州本地柚光青及其他地区普通柚,且受年份和环境的影响较大;随着果径的增大,2个化州柚种质正毛(吴)和假西洋(吴)果皮及果肉中柚皮苷含量均呈现下降趋势,且果肉中柚皮苷含量比果皮中高。综合分析果实大小和有效成分含量得出,果实直径4~8 cm时是化州柚适宜采收期,果径6 cm左右为最适采果期。
英文摘要:
      Simple sequence repeat (SSR) markers were used to study the genetic relationship among different strains of Citrus grandis ‘Tomentosa’ and other representative germplasms of Citrus. 175 alleles were amplified with 25 pairs of SSR primers and 15 samples. The results of cluster analyses showed that ‘Tomentosa’ and pomelos were closely related. Some ‘Tomentosa’ materials with homonym are found to be genetically different. High performance liquid chromatography (HPLC) was used to determine the content of naringin in young fruit of different germplasm and fruit tissue of ‘Tomentosa’ with varied diameters. The results showed that the content of naringin in the young fruit of ‘Tomentosa’ was significantly higher than that of Huazhou local pomelo ‘Guangqing’ and common pomelo from other areas,affected by the growing season and environmental factors. With the increase of fruit diameter,the content of naringin in pericarp and pulp of ‘Zhaomao(Wu)’ and ‘Jiaxiyang(Wu)’ decreased,and the content of naringin in pulp was higher than that in pericarp. The results of comprehensively analyzing fruit size and active ingredient content showed that the fruit diameter of 4-8 cm was the suitable harvesting period of ‘Tomentosa’,and the fruit with diameter of 6 cm was optimal for harvesting.
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