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刘晴,刘宇彤,邓世林,鲍彤,董爱荣.短密木霉降解咪唑乙烟酸候选基因的筛选[J].华中农业大学学报,2019,38(4):
短密木霉降解咪唑乙烟酸候选基因的筛选
Screening candidate genes of degradating imazethapyr by Trichoderma brevicompactum
投稿时间:2019-01-16  
DOI:
中文关键词:  短密木霉  咪唑乙烟酸; 转录组; 蛋白质组学; 关联分析
英文关键词:Trichoderma brevicompactum  imazethapyr  transcriptome  proteome  association analysis
基金项目:国家自然科学基金项目(31670494)
作者单位E-mail
刘晴 东北林业大学林学院哈尔滨 150040 401339721@qq.com 
刘宇彤 东北林业大学林学院哈尔滨 150040  
邓世林 东北林业大学林学院哈尔滨 150040  
鲍彤 东北林业大学林学院哈尔滨 150040  
董爱荣 东北林业大学林学院哈尔滨 150040 darlmy@163.com 
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中文摘要:
      为探明短密木霉(Trichoderma brevicompactum)降解咪唑乙烟酸的分子机制,通过转录组学和蛋白质组学数据整合分析筛选降解咪唑乙烟酸的候选基因。结果显示:以500 mg/kg咪唑乙烟酸驯化后的短密木霉为材料,分别在基础培养基和以咪唑乙烟酸为单一碳源的培养基中培养至降解效率最高的第7天,在转录组学中差异表达的基因 1 329个,其中上调703个,下调626个。用ITRAQ进行蛋白质组学分析,共鉴定到21 883条肽段和4 003个蛋白质。关联分析中,鉴定出24个在转录组和蛋白质组中均差异表达的基因,其中14个基因上调,10个基因下调。定量蛋白质和基因的表达关联系数为-0.047 2,显著差异蛋白质和显著差异基因表达关联系数为-0.142 0,蛋白质和表达变化趋势相同的基因关联系数为-0.741 3,蛋白质和表达变化趋势相反的基因关联系数为-0.7916。关联分析鉴定的24个基因中,有2个基因具有功能注释,分别为TBU3981A(NCBInr:绿色木霉Gv29 8糖苷水解酶16家族部分mRNA)和TBU1425A(NCBInr:HEX 1),可作为降解咪唑乙烟酸的候选基因。
英文摘要:
      The candidate genes of degradating imazethapyr were screened with integration analyses of transcriptomics and proteomics data to explore the molecular mechanism of degradating imazethapyr in Trichoderma brevicompactum. The results showed that when the basal medium with 500 mg/kg mazethapyr was used as the sole carbon source to cultivate T. brevicompactum for seven days, the highest degradation efficiency was obtained. There were 1 329 genes with significant transcriptomic differences, of which 703 were up regulated and 626 were down regulated. 21 883 peptides and 4 003 proteins were identified by proteome analyses performed with ITRAQ. The results of association analyses showed that 24 genes with differential expression and protein abundance were correspondingly changed, of which 14 were up regulated and 10 were down regulated. The association coefficient between quantitative protein and gene expression was -0.047 2. The association coefficient between significant difference protein and genes with significantly differential expression was -0.142 0, with the similar trend of expression change. The association coefficient between protein and gene expression was -0.741 3.The association coefficient between protein and genes with the opposite trend of expression change was -0.791 6. Two of the 24 genes identified by association analysis had functional annotations. They are Trichoderma virens Gv29 8 glycoside hydrolase family 16 protein partial mRNA and HEX 1 gene, respectively. They can be used as candidate genes of degradating imazethapyr. It is indicated that the candidate genes of degradating imazethapyr by T. brevicompactum can be effectively screened with the association analyses of transcriptome and proteome.
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