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孙千惠,田万平,罗航,王卫民,刘红.团头鲂hepcidin基因单核苷酸多态性及与抗病性状的相关性[J].华中农业大学学报,2019,38(2):38-44
团头鲂hepcidin基因单核苷酸多态性及与抗病性状的相关性
Single nucleotide polymorphisms and its association with disease resistant trait of hepcidin gene in Megalobrama amblycephala
投稿时间:2018-10-30  
DOI:
中文关键词:  团头鲂  铁调素基因  SNPs  抗病  嗜水气单胞菌
英文关键词:Megalobrama amblycephala  hepcidin gene  SNPs  disease resistance  Aeromonas hydrophila
基金项目:国家自然科学基金项目(31572613); 中央高校基本科研业务费专项(2662015PY134)
作者单位E-mail
孙千惠 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉 430070 2504337931@qq.com 
田万平 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉 430070  
罗航 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉 430070  
王卫民 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉 430070  
刘红 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉 430070 liuhong59@mail.hzau.edu.cn 
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中文摘要:
      为了解hepcidin(铁调素)基因的多态性及其与抗病性状的相关性,以团头鲂(Megalobrama amblycephala)为研究对象,从公共数据库中获得hepcidin基因序列,对团头鲂进行嗜水气单胞菌(Aeromonas hydrophila)人工感染来区分易感和抗病个体,采用PCR扩增及测序技术筛选hepcidin基因序列中的SNP(单核苷酸多态,single nucleotide polymorphisms)位点,对SNP位点运用高分辨率熔解曲线法(high resolution melting,HRM)进行分型,分析其多态性及与抗病性状之间的关系。结果在hepcidin基因上共筛选出2个SNP位点,其中1个位点位于内含子部分,1个位于3′非编码区。经过统计分析发现,位于3 371 bp(A/G)的这一SNP位点的基因型频率和等位基因频率在易感和抗病组间差异极显著,位于217 bp(A/G)位点的基因型频率和等位基因频率在易感组和抗病组间差异显著。据此推测hepcidin的这2个SNP位点多态性与团头鲂抗病性状相关。
英文摘要:
      The genomic sequences of hepcidin in Megalobrama amblycephala were obtained from public database to study the association between the hepcidin gene polymorphism and disease resistance trait. Aeromonas hydrophila challenge experiment was used to distinguish susceptible and disease resistant M. amblycephala individuals.The single nucleotide polymorphisms (SNP) of the hepcidin gene in M. amblycephala were screened with PCR and DNA sequencing. Highresolution melting (HRM) were used for SNP genotyping. Two SNPs of the hepcidin gene were screened,one of which was located in the intron region and the other was located in the 3′untranslated region. The results of statistical analyses showed that the genotype and allele frequencies of SNP (A/G) located at 3 371 bp was extremely significant different (P<0.01) and SNP (A/G) located at 217 bp was significant different (P<0.05) between the susceptible and resistant group. It is indicated that the polymorphism of hepcidin gene was significantly associated with bacterial septicemia resistance.
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