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陈柏湘,王伟峰,王卫民,王焕岭.团头鲂低氧耐受相关SNPs标记的开发[J].华中农业大学学报,2019,38(2):23-29
团头鲂低氧耐受相关SNPs标记的开发
Isolation of SNP markers associated with hypoxia tolerance in Megalobrama amblycephala
投稿时间:2018-11-05  
DOI:
中文关键词:  团头鲂  低氧  SNP  性状关联分析
英文关键词:Megalobrama amblycephala  hypoxia  SNP  trait association
基金项目:国家自然科学基金项目(31872542);中央高校基本业务费专项(2013PY067)
作者单位E-mail
陈柏湘 1.华中农业大学水产学院武汉 430070 2.广东佛山市南海百容水产良种有限公司佛山 5 155290338@qq.com 
王伟峰 华中农业大学水产学院武汉 430070  
王卫民 华中农业大学水产学院武汉 430070  
王焕岭 华中农业大学水产学院武汉 430070 huanlingok@mail.hzau.edu.cn 
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中文摘要:
      为寻找耐低氧相关SNPs分子标记辅助团头鲂优良品种选育,利用团头鲂低氧耐受和敏感群体的转录组数据,进行SNPs位点的开发和鉴定,获得52 623个可能的SNPs位点,其中碱基转换和颠换型位点分别占57.4%(30 192个)和31.9%(16 802个);同义SNPs占编码区总SNPs的99.7%,非同义SNPs仅有32~35个;使用PCR RFLP技术对筛选出的5个非同义的多态性SNPs进行耐低氧性状关联分析。结果显示,Plin2 A1157G和Hif 3α A2917G位点的SNPs在团头鲂亲本群体中与低氧耐受性状显著相关,但在子代群体中却与低氧耐受性状无显著相关性。这说明亲代中筛选出的分子标记并不一定适用于子代群体,在利用分子标记辅助育种时需要考虑标记在子代中的有效性。
英文摘要:
      In order to obtain SNP markers associated with hypoxia tolerance trait for applying in molecular marker assisted breeding of Megalobrama amblycephala,in the present study,based on the constructed transcriptome data in hypoxia tolerant and sensitive M. amblycephala,52 623 potential SNPs including transition type of 30 192 (57.4%) and transversion type of 16 802 (31.9%) were found. Synonymous SNPs accounted for 99.7% of total SNPs located in the coding region,while only 32~35(0.3%) nonsynonymous SNPs were found. Five nonsynonymous and polymorphic SNPs were obtained using PCR-RFLP method. Analysis showed that two SNPs,Plin2 A1157G and Hif3α A2917G,were significantly associated with hypoxia tolerance trait in the parental populations. But further analysis revealed that no significant association existed between the two SNPs and hypoxia tolerance trait in the offspring populations. It is indicated that molecular markers isolated in the parent populations may be inappropriate in their offspring,therefore,the effectiveness of markers in offspring needs to be considered in molecular marker assisted breeding.
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