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邰志鹏,徐异桓,张电光,谭肖英.黄颡鱼bmp2a与bmp4基因启动子的克隆及分析[J].华中农业大学学报,2019,38(1):91-96
黄颡鱼bmp2a与bmp4基因启动子的克隆及分析
Molecular cloning and analysis of bmp2a and bmp4 promoters in yellow catfish Pelteobagrus fulvidraco
投稿时间:2018-06-30  
DOI:
中文关键词:  黄颡鱼  骨形态形成蛋白  启动子  转录因子
英文关键词:Pelteobagrus fulvidraco  bone morphogenesis protein,BMP  promoter  transcription factor
基金项目:国家自然科学基金项目( 31572605,31001101)
作者单位E-mail
邰志鹏 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉430070 422413099@qq.com 
徐异桓 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉430070  
张电光 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉430070  
谭肖英 华中农业大学水产学院/农业农村部淡水生物繁育重点实验室武汉430070 txy7933@mail.hzau.edu.cn 
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中文摘要:
      为研究黄颡鱼bmp2a与bmp4基因的功能及其相关转录因子的调控网络,采用RLM 5′ RACE方法确定bmp2a与bmp4基因的转录起始位点,通过hiTAIL PCR方法克隆bmp2a与bmp4基因启动子序列并运用生物信息学方法预测启动子序列上的关键转录因子结合位点。结果显示,bmp2a与bmp4基因的转录起始位点分别位于bmp2a与bmp4基因编码区上游的391 bp与351 bp处。克隆bmp2a与bmp4的1 830 bp、1 962 bp启动子序列,在启动子序列的基础上预测出AP1、SP1、E box、GATA1、CREB、PPARγ、SOX5、SOX6和SOX9等转录因子的结合位点,提示这些转录因子对bmp2a与bmp4基因的转录调控发挥潜在的重要作用。
英文摘要:
      To explore the function of the bmp2a and bmp4 genes and regulatory networks of the related transcription factors in yellow catfish Pelteobagrus fulvidraco, RLM 5′RACE method was used to identify the transcription start site (TSS) of bmp2a and bmp4. Then, the promoters of bmp2a and bmp4 were cloned by hiTAIL PCR method and a cluster of putative binding sites of several transcription factors were identified by bioinformatics analysis. The results showed that the TSS of bmp2a and bmp4 were located at 391 bp and 351 bp upstream of the coding sequence, respectively. Then 1 830 bp and 1 962 bp upstream of the TSS of bmp2a and bmp4 were cloned and key binding sites of several transcription factors, such as AP1, SP1, GATA1, CREB, PPARγ, SOX5, SOX6 and SOX9, were predicted, suggesting that these transcription factors may play crucial roles in the transcriptional regulation of the bmp2a and bmp4 genes.
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