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胡洪涛,曹春霞,闵勇,张舒,夏贤格,汪本福,张佑宏.秸秆腐熟剂对稻麦轮作区土壤微生态的影响 [J].华中农业大学学报,2018,37(06):45-57
秸秆腐熟剂对稻麦轮作区土壤微生态的影响 
Effects of straw compositing agent on soil microbial ecology in rice-wheat rotation field
投稿时间:2018-04-16  
DOI:
中文关键词:  稻麦轮作  秸秆还田  秸秆腐熟剂  土壤微生态  微生物组  微生物多样性
英文关键词:rice-wheat rotation  straw incorporation  straw compositing agent  soil microbial ecology  microbiome  microbial diversity
基金项目:国家重点研发计划项目(2016YFD0200807);湖北省农业科学院重大成果培育专项(2017CGPY01);湖北省农业创新团队项目(2016-620-000-001-039)
作者单位E-mail
胡洪涛 农业农村部农业废弃物肥料化利用重点实验室武汉 430064湖北省生物农药工程研究中心武汉 430064 hzh0005@qq.com 
曹春霞 湖北省生物农药工程研究中心武汉 430064  
闵勇 农业农村部农业废弃物肥料化利用重点实验室武汉 430064湖北省生物农药工程研究中心武汉 430064  
张舒 湖北省农业科学院植保土肥研究所武汉 430064 ricezs6410@163.com 
夏贤格 农业农村部农业废弃物肥料化利用重点实验室武汉 430064 13607123150@139.com 
汪本福 湖北省农业科学院粮食作物研究所武汉 430064  
张佑宏 湖北省生物农药工程研究中心武汉 430064  
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中文摘要:
      采用核糖体RNA基因高通量测序技术,研究在宜城稻麦轮作田施用4种不同秸秆腐熟剂处理(T1:棘孢木霉/枯草芽胞杆菌粉剂;T2:黑曲霉/枯草芽胞杆菌粉剂;T3:黑根霉/枯草芽胞杆菌粉剂;T4:棘孢木霉/枯草芽胞杆菌粉剂)对土壤微生态的影响。结果显示:各处理对土壤真菌种群影响较大,相对于阴性对照(Tn),科和属的数量均明显增加,以T2处理增幅最大(133.0%);与阳性对照(Tp)比较,除T4处理外,各处理科或属的数量也有所增加(15.6%~45.8%);然而,细菌种群并未见较大变化。Chao1指数比较显示,真菌指数大幅增加(74.5%~191.8%),而细菌的指数变化较小(-4.0%~15.1%)。统计分析显示,各处理有24~55个属的真菌显著性上调( P <0.05和倍数变化≥2),其中超过六成相对于Tp和Tn均上调,而仅有2~11个属的细菌显著上调;有4~11个属的真菌显著性下调,其中14.3%~25.0%相对于2个对照均下调,有4~7个属的细菌相对于Tp显著下调,但没有属相对于Tn下调。通过UPGMA聚类分析不同处理土壤微生物Beta多样性之间的相似度,土壤真菌Beta多样性结果显示,T1、T2、T3和阳性对照均在一个分组里,而T4和阴性对照在一个分组里;土壤细菌Beta多样性结果显示,T2、T3、T4和阳性对照均在一个分组里,而T1和阴性对照在一个分组里。
英文摘要:
      Microbiome sequencing technology was used to study the effects of applying 4 kinds of straw compositing agents including T1( Trichiderma asperellum/Bacillus subtilis ),T2( Aspergillus niger/Bacillus subtilis ),T3( Rhizopus nigricans / Bacillus subtilis ) and T4( Trichiderma asperellum / Bacillus subtilis ) on soil microbial ecology.The results showed that the population of fungi was more affected by the treatments,in which the number of family and genus was obviously increased.The number of family and genus in the T2 treatment was the biggest (133.3%) compared to positive control (Tp).The number of family or genus in the T4 treatment increased with a small range from 15.6%-45.8%.The population of bacteria was not largely changed.Results of comparing Chao1 index showed that the fungi index dramatically increased with a range from 74.5% to 191.8%,whereas bacteria index was changed at a small scale (-4.0%-15.1%).Results of statistics analyses showed that 24 to 55 genera of fungi in different treatments were significantly up-graded ( P <0.05 and fold changes≥2),of which more than 60% were commonly significantly up-graded in contrast to both Tp and Tn,in which only 2-11 genera of bacteria were significantly up-graded.4-11 genera of fungi were significantly down-graded,of which 14.3%-25.0% were commonly significantly up-graded in contrast to both Tp and Tn,in which 4-7 genera of bacteria were significantly down-graded related to Tp,but none was down-graded compared to Tn.The similarities of microorganism Beta diversity in different treatments were analyzed by UPGMA cluster.The results of soil fungal Beta diversity showed that T1,T2,T3 and positive control were in the same cluster,while T4 and negative control in another cluster. The results of bacterial Beta diversity showed that T2,T3,T4 and positive control were in the same cluster,while T1 and negative control were in the same cluster.
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