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胡甜,汪方奎,张少然,陈雯莉.常见细菌培养物光密度与浓度的关系研究[J].华中农业大学学报,2018,37(06):40-44
常见细菌培养物光密度与浓度的关系研究
Relationship between absorbance and bacterial density
投稿时间:2018-05-12  
DOI:
中文关键词:  细胞浓度  流式细胞术  分光光度法  光密度  细胞数目
英文关键词:cell concentration  flow cytometry  spectrophotometry  absorbance  cell number
基金项目:国家自然科学基金项目(31570048)
作者单位E-mail
胡甜 华中农业大学农业微生物学国家重点实验室武汉 430070 2466475678@qq.com 
汪方奎 华中农业大学农业微生物学国家重点实验室武汉 430070  
张少然 华中农业大学农业微生物学国家重点实验室武汉 430070  
陈雯莉 华中农业大学农业微生物学国家重点实验室武汉 430070 wlchen@mail.hzau.edu.cn 
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中文摘要:
      为建立准确评估细菌培养物浓度和生长状态的方法,分别采用流式细胞仪和分光光度计对实验室常见3种培养物包括大肠杆菌 Escherichia coli S17-1、集胞蓝细菌 Synechocysitis sp. PCC 6803和枯草芽胞杆菌 Bacillius subtilis 168在不同浓度下的细胞数目和光密度进行了测定。随后将每个培养物在系列浓度下的光密度和细胞浓度进行比较,建立了光密度-细胞浓度的函数关系。结果表明,在一定浓度下,3种实验室常见培养物的光密度和细胞浓度呈显著线性正相关。最后,采用拟合函数验证在培养过程中细菌培养物的光密度与细胞浓度的关系。本研究建立了 Escherichia coli S17-1、 Synechocysitis sp. PCC 6803和 Bacillius subtilis 168 菌株光密度与细胞浓度的关系,可为相关菌株采用分光光度法判断培养物细胞浓度及下游分子生物学实验的开展奠定基础。
英文摘要:
      Flow cytometry and spectrophotometry were used to measure the cell numbers and absorbances of three common cultures including Escherichia coli S17-1, Synechocystis sp. PCC 6803 and Bacillius subtilis 168 at different concentrations in the laboratory. Then the absorbance values and cell numbers of each culture were compared. The function relationship between absorbance and cell numbers was established. The results showed that the absorbance value and cell numbers of the three common cultures in the laboratory were significantly positively correlated with the concentration at the certain concentration. It was verified that the absorbance value of the culture can reflect the number of cells during the culture process using the fitting function. The relationship between the absorbance and cell number of three strains including Escherichia coli S17-1, Synechocystis sp. PCC 6803 and Bacillius subtilis 168 was established. It will provide the foundation for developing biological experiments to determine the cell number and downstream molecules in the culture using a spectrophotometer. 
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