Abstract:Based on the previous droughtassociatedgeneenriched SSHcDNA library and cDNAmicroarray,we preliminarily screened an HuABAR Unigene presumably involving in the tolerance of abiotic stresses including drought,cold and high temperature.Fulllength cDNA sequence of this gene was cloned.Bioinformatic analyses and subcellular localization was carried out.The results showed that HuABAR gene was significantly upregulated in response to abiotic stresses including in drought,high temperature and low temperature.The highest expression level was observed at the 5th day or the third day after drought stress or high temperature stress.The expression was increasingly unregulated as exposure to low temperature within five days.HuABAR gene contained 1 239 bp in full cDNA length,and consisted of 264 bp 5′UTR,414 bp 3′UTR,and 561 bp open reading frame (ORF) encoding 187 amino acids.This gene might encode a typical SRPBCC domain,and was highly similar to the PYR1/PYLs (pyrabactin resistance 1/PYR1 like) family.The target gene was ligated with green fluorescent protein (GFP) by constructing plant transient expression vector pBWA (V) HSHuABARgfp,and the PEGmediated method was used to transiently transform into Arabidopsis protoplasts.It was found that target gene was located in the cytoplasm,which satisfied the expectations.It is indicated that HuABAR in pitaya may be involved tolerance of multiple abiotic stresses.