5种经济鱼类基因组大小的测定
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四川省科技厅应用基础项目(2015JY0262); 四川省教育厅重点项目(15ZA0285);四川省大学生创新项目(16NSD-47,201710640023)


Genome size determination of five commercial fishes 
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    摘要:

    采用流式细胞术,以鸡(Gallus gallus)血细胞 DNA 含量(2.3 pg/2C,C指单倍体)为标准,对5种经济鱼类白甲鱼(Onychostoma sima)、三倍体兴国红鲤(triploid Cyprinus carpio var. singuonensis)、胭脂鱼(Myxocyprinus asiaticus )、中华沙鳅(Sinibotia superciliaris)和花鱼骨 (Hemibarbus maculatus)的基因组大小(或称C-值)和倍性进行测定。结果表明,5种淡水鱼类的的C-值分别为:白甲鱼(1.18±0.01) pg,三倍体兴国红鲤(1.92±0.07) pg,胭脂鱼(2.62±0.28) pg,中华沙鳅(0.89±0.01) pg,花鱼骨 (1.24±0.02) pg,C-值从大到小排列依次为:胭脂鱼>兴国红鲤>花鱼骨 >白甲鱼>中华沙鳅。在5种经济鱼类中,除了亲缘关系很近的白甲鱼与花鱼骨 (同一科)的C-值大小差异不显著外(P>0.05),其余的种间(不同科)的C-值大小差异显著。倍性分析结果表明,白甲鱼、花鱼骨 和中华沙鳅属于二倍体,兴国红鲤属于三倍体,胭脂鱼属于四倍体。

    Abstract:

    In this paper,the genome size (C-value) and ploidy of 5 commercial fishes including Onychostoma sima,triploid Cyprinus carpio var. singuonensis,Myxocyprinus asiaticus,Sinibotia superciliarisa and Hemibarbus maculatus were determined by flow cytometry using chicken erythrocyte DNA content as a standard. The results showed that the C-values of O. sima,triploid C. carpio var. singuonensis,M. asiaticus,S. superciliarisa and H. maculatus were 1.18±0.01 pg,1.92±0.07 pg,2.62±0.28 pg,0.89±0.01 pg and 1.24±0.02 pg,respectively. The C-value of M. asiaticus was the largest,followed by triploid C. carpio var. singuonensis, H. maculatus,O. sima and S. superciliarisa. Among the 5 economic fishes,there is no difference in C-values between O. sima and H. maculatus (the same family) (P>0.05),but the difference between the other species (different families) was significant (P<0.05). Ploidy analysis showed that O. sima,S. superciliarisa and H.maculatus were diploid,C. carpio var. singuonensis was triploid and M. asiaticus was tetraploid.

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邹远超,符鹏,李骞,吴田,王永明,谢碧文.5种经济鱼类基因组大小的测定[J].华中农业大学学报,2018,37(3):87-91

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  • 在线发布日期: 2018-05-03
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