Crucian carp,grass carp,bream and yellow catfish were used to investigate the force conditions for live fish under the process of transportation and obtain force curve of transportation for live fish through testing conditions of different road and vehicle including straight line driving,over deceleration belt and uniform circular motion.The force value curve was filtered and the values of mechanical characteristics were extracted and analyzed.The results showed that there was a significant difference of affecting values of partial mechanical characteristics under the different kinds of fish and speed of vehicles and speed of acceleration.Under conditions of the three different driving,uniform circular motion had the greatest impact on the maximum force.It will provide a foundation for studying stress mechanism of live fish carried by vehicles under conditions of different transportation.