Abstract:In order to improve the indoor reproductive efficiency of Habrobracon hebetor,effects of mating experience and sex ratio of parent wasps on mating behavior and offspring sex allocation were investigated by controlling the mating condition indoors. Male mating experience had significant effect on the mating behavior of wasps. Male wasps with less mating experience showed more courtship frequency,stronger courtship intensity,more mating frequency and longer mating duration at next mating behavior. The recopulation duration of male wasp without mating experience was significantly longer than that of male wasp with 5 to 6 mating experiences. Male mating experience showed some impacts on the offspring sex ratio,but no obvious impact on the total number of the offspring. When the male with fewer mating experience mated with virgin female,more femalebiased offspring sex ratios were obtained. For indoor rearing,the number of female offspring could be improved by enhancing proportion of the male parent. The best propagating efficiency of H. hebetor was obtained when the sex ratio of parent wasp was 1 female to 3 male,and the ratio of natural enemy to pests was 2 female wasps to 10 5th instar larvae of Plodia interpunctella.