麦蛾气味受体OrCo基因的克隆及其表达模式分析
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中央高校基本科研业务费专项(2662015JC008;2662017JC032)


Cloning and expression profile analysis of odorant receptor gene in Sitotroga cerealella
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    摘要:

    采用RTPCR和RACE技术,克隆了麦蛾OrCo基因全长序列,并将该基因命名为ScerOrCo。序列分析结果显示,ScerOrCo全长为1 876 bp,开放阅读框长1 422 bp,编码473个氨基酸,序列中有7个跨膜区。ScerOrCo基因的氨基酸序列与粘虫Mythimna separate嗅觉受体的同源性高达87%。qRTPCR检测结果表明ScerOrCo只在麦蛾触角中特异性表达,且雌雄虫间表达量无显著差异。

    Abstract:

    In the present study,the fulllength cDNA of the odorant receptor coreceptor gene was cloned from the antennae of Sitotroga cerealella (named ScerOrCo) by using reverse transcription polymerase chain reaction (RTPCR) and rapid amplification of cDNA ends (RACE).Sequence analysis revealed that the transcript consisted of 1 876 nucleotides and the open reading frame was 1 422 bp,encoding a peptide of 437 amino acids with 7 putative transmembrane domains.The deduced amino acid sequence of ScerOrCoshared the highest similarity with the olfactory receptor of Mythimna separate (87%).The results of quantitative real time RTPCR showed that the ScerOrCo mRNA was expressed specifically in adult antennae and had no difference between female and male (P>0.05).

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应益益,马敏,陆艳,刘灿,李雪刚,杨凤连.麦蛾气味受体OrCo基因的克隆及其表达模式分析[J].华中农业大学学报,2018,37(1):46-51

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  • 收稿日期:2017-07-31
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  • 在线发布日期: 2018-01-02
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