Fractions with high inhibitory activities on α-amylase and lipase were isolated from water extracts of Qingzhuan tea via ultra-filtration technology.Results showed that the trapped fraction of 5 ku ultra-filtration isolated from water extracts had strong α-amylase inhibitory activity.The trapped fraction of 100 ku ultra-filtration isolated from water extracts had strong lipase inhibitory activity.The α-amylase and lipase inhibitory activity of trapped fraction of 5 ku ultra-filtration isolated from the ethyl acetate fraction was higher than that of the ethyl acetate fraction.It is indicated that ultra-filtration combining with ethyl acetate extraction can more effectively isolate fractions with high α-amylase and lipase inhibitory activities from water extracts of Qingzhuan tea.