To explore the metabolicmechanisms of rosette induced by high temperature,GC-MS was used to study metabolic differences in mature leaves and shoot tips of Eustoma grandiflorum under normal (22 ℃) and high (30 ℃) temperatures.Thirty metabolites were identified and results of the PLS DA analyses of them showed that the metabolites in different organs under different temperatures were separated clearly.Analyses of variance showed that many sugars were decreased and organic acids increased significantly in shoot tips under the high temperature compared with that under the normal temperature.The case in mature leaveswas completely opposite.It is indicated that under the high temperature,the decrease of sugars in shoot apex caused the rosette,while the accumulation of sugars in mature leaves improved the stress resistance of Eustoma grandiflorum.The metabolite difference between mature leaf and shoot tip under the high temperature is less than that under the normal temperature.It is suggested that the larger difference under the normal temperature shows the different development direction between leaf and shoot,while the smaller difference implies the systematic mechanism of Eustoma grandiflorum in response to abiotic stress.