水稻花青素合成调控转录因子 OsC1 和 OsPAC1的功能
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中央高校基本科研业务费专项(2013PY062)


Function of transcription factor OsC1 and OsPAC1 regulating biosynthesis of rice anthocyanin
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    摘要:

    从黑米品种黑帅中克隆水稻内源调控花青素合成的转录因子OsC1和OsPAC1,并导入到白米粳稻品种Chao 210和ZH11中超量表达,结果显示:在超量表达OsC1的Chao 210的抗性愈伤分化阶段、再生转基因植株的叶片、叶鞘、稃尖等部位观察到花青素的积累;其他转基因材料,如超量表达 OsC1的ZH11、超量表达OsPAC1的Chao 210和ZH11均未观察到花青素的积累;利用高效液相色谱(HPLC)测定转基因水稻叶片中花青素含量,发现主要是矢车菊素3O葡萄糖苷和芍药素3-O-葡萄糖苷2种花青素。对转基因材料中花青素生物合成相关基因的表达量进行分析发现,Chao 210和ZH11超量表达OsC1后,花青素代谢途径上的大部分结构基因上调表达;Chao 210中超量表达OsPAC1后,OsCHS、OsCHI、OsANS、OsUFGT上调表达,而OsF3H、OsF3’H表达基本没变;ZH11中超量表达OsPAC1后,OsCHS、OsANS、OsUFGT上调表达,而OsCHI、OsF3H、OsF3’H的表达基本不变。

    Abstract:

    Rice endogenous transcription factor OsC1 and OsPAC1, which may regulate the biosynthesis of anthocyanidin,were isolated from black rice Heishuai and overexpressed in white rice Chao 2 10 and ZH11.Anthocyanin accumulation was observed in the resistant callus,leaves,leaf sheath,apiculus of OsC1 overexpressed Chao 210.Results of high performance liquid chromatography (HPLC) analysis showed that anthocyanins cyanidin 3- O-glucoside and peonidin 3-O-glucoside were accumualted in OsC1 overexpressed Chao 210.No anthocyanin accumulation were observed in other transgenic rice materials including OsC1 overexpressed Chao 2-10,OsC1overexpressed ZH11 and OsPAC1 overexpressed ZH11.Expression analysis of structural genes related to the pathway of anthocyanin biosynthesis in transgenic rice showed that most of the structural genes involved in anthocyanin biosynthesis were upregulated in OsC1 overexpressed Chao 210 and ZH11 exceptOsDFR.Expressions of OsCHS,OsCHI,OsANS and OsUFGT were up regulated in OsPAC1 overexpressed Chao 2 10,but the expression levels of OsF3H and OsF3′H were not changed.Expressions of OsCHS,OsANS and OsUFGT were up regulated in OsPAC1 overexpressed ZH11.The expression level of OsCHI,OsF3H,and OsF3′H kept unchanged.

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李艳霞,林拥军,陈浩.水稻花青素合成调控转录因子 OsC1 和 OsPAC1的功能[J].华中农业大学学报,2017,36(1):1-9

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  • 收稿日期:2016-04-15
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  • 在线发布日期: 2016-12-29
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